Mcf 7

Human colon cancer cells SW480 (ATCC # CCL-228™) were obtained from the American Type Culture Collection (ATCC, USA). Human colon cancer cells (HCT116, Lovo), human breast cancer cells (MCF-7), human hepatoma carcinoma cells (SMMC-7721), human hepatic cells (LO2) and african green monkey kidney kidney cells (Vero) were purchased from BOSTER (Wuhan, China) and cultured in high Glucose Dulbecco’s modified Eagle’s medium supplemented with 2 mM glutamine, 10% (v/v) fetal bovine serum (FBS), 100 U/mL penicillin and 100 mg/mL streptomycin. Cell cultures were grown and maintained at 37 °C in the humidified tissue culture incubator with 5% CO₂ using standard tissue procedures.

The breast cancer cell line (MCF-7) and normal mammary epithelial cell line (MCF-10A) were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences. MCF-7 cells were cultured in DMEM (Boster, Wuhan, China) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Gibco, CA, United States). MCF-10A cells were kept in DMEM/F12 (Procell, Wuhan, China) supplemented with 20 ng/ml epidermal growth factor, 0.5 μg/ml hydrocortisone, 5% horse serum and 1% penicillin/streptomycin. Both cell lines were maintained in a humidified incubator at 37°C with 5% CO₂.
Small interfering RNA (siRNA) targeting circ_0008812 (si-circ8812: ACG​AGU​GCA​CUU​GGU​GAA​AUU), circ_0001583 (si-circ1583: CAA​AGA​AGG​CCA​AGG​UUA​AUU) and negative control (si-NC) were generated by Tsingke Biotechnology (Chengdu, China). MCF-7 cells were transfected using Lipofectamine 3000 (Invitrogen) for 48 h.