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Ret 3 rectal probe for mice

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The RET-3 rectal probe is a laboratory equipment used to measure the body temperature of mice. The probe is designed to be inserted into the rectum of the animal to provide accurate temperature readings.

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Lab products found in correlation

Bat 12 microprobe thermometer, by Physitemp (3 mentions) Cl316 243, by Merck Group (1 mentions) Bat 12, by Physitemp (1 mentions)

9 protocols using ret 3 rectal probe for mice

1

Rectal Temperature Measurement in Mice

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Temperature was monitored daily (~18:00 EST) using a TH-5 Thermalert Clinical Thermometer (Physitemp) attached to a RET-3 rectal probe for mice (Physitemp). The probe was lubricated with glycerol and was inserted 1.27 centimeters (1/2 inch) and temperature was measured when stabilized.
Benvie A.M., Lee D., Steiner B.M., Xue S., Jiang Y, & Berry D.C. (2023). Age-dependent Pdgfrβ signaling drives adipocyte progenitor dysfunction to alter the beige adipogenic niche in male mice. Nature Communications, 14, 1806.
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2

Metabolic Profiling of Cold Exposure

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Fat and lean mass was determined by echoMRI-100V. CT Scan was performed on Trifoil eXplore RS9 microCT system. Oxygen consumption was measured using the Comprehensive Laboratory Animal Monitoring System (CLAMS). Data were normalized to lean body mass determined by EchoMRI. Mice were individually caged and maintained under a 12 hr light/12 hr dark cycle. Food consumption and locomotor activity were tracked. Cold exposed mice were housed for 10 days at thermoneutrality before cold exposure at 4°C. Body temperatures were assessed using a RET-3 rectal probe for mice (Physitemp). CL316,243 (Sigma) was intraperitoneally injected into mice at 1mg/kg. FLIR E5 series infrared camera was employed to capture thermography.
Nguyen H.P., Yi D., Lin F., Viscarra J.A., Tabuchi C., Ngo K., Shin G., Lee A.Y., Wang Y, & Sul H.S. (2020). Aifm2, a NADH oxidase, supports robust glycolysis and is required for cold- and diet-induced thermogenesis. Molecular cell, 77(3), 600-617.e4.
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3

Rectal Temperature Measurement in Mice

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Rectal temperature was measured in mice with a BAT-12 Microprobe Thermometer with a RET3 rectal probe for mice (Physitemp Instruments, Inc., Clifton, NJ). Additional details are provided in the Supplemental Information.
Mitchell S.J., Matute J.M., Scheibye-Knudsen M., Fang E., Aon M., González-Reyes J.A., Cortassa S., Kaushik S., Gonzalez-Freire M., Patel B., Wahl D., Ali A., Calvo-Rubio M., Burón M.I., Guiterrez V., Ward T.M., Palacios H.H., Cai H., Frederick D.W., Hine C., Broeskamp F., Habering L., Dawson J., Beasley T.M., Wan J., Ikeno Y., Hubbard G., Becker K.G., Zhang Y., Bohr V., Longo D.L., Navas P., Ferrucci L., Sinclair D.A., Cohen P., Egan J.M., Mitchell J.R., Baur J.A., Allison D.B., Anson R.M., Villalba J.M., Madeo F., Cuervo A.M., Pearson K.J., Ingram D.K., Bernier M, & de Cabo R. (2016). Effects of Sex, Strain, and Energy Intake on Hallmarks of Aging in Mice. Cell metabolism, 23(6), 1093-1112.
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4

Assessing Mouse Cold Exposure

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Body temperatures were assessed in 4-month-old mice using a RET-3 rectal probe for mice (Physitemp). For the cold exposure experiment, mice were housed individually and transferred to a cold room with an ambient temperature of 4 °C. Temperature was measured every 30 min for 4 h, or until mouse core body temperature dropped below 25° C.
Chennamsetty I., Coronado M., Contrepois K., Keller M.P., Carcamo-Orive I., Sandin J., Fajardo G., Whittle A.J., Fathzadeh M., Snyder M., Reaven G., Attie A.D., Bernstein D., Quertermous T, & Knowles J.W. (2016). Nat1 deficiency is associated with mitochondrial dysfunction and exercise intolerance in mice. Cell reports, 17(2), 527-540.
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5

Hypothermia Response to DPAT in DS and WT Mice

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Separate groups of DS and WT mice at P18 and P35 were monitored for hypothermia in response to a single acute injection of (+/−)-8-hydroxy-2-(dipropylamino)tetralin hydrobromide (DPAT) (0.5 mg/kg). For this a flexible temperature probe (RET-3 rectal probe for mice, Physitemp Instruments, Clifton New Jersey) was carefully inserted rectally and the mouse was given about 5 minutes to adapt to the probe and the chamber. Baseline temperature was recorded and an injection of DPAT was given. Following the injection temperature was continuously followed for 15 minutes, when mice were returned to their home cage.
Hatini P.G, & Commons K.G. (2019). Serotonin Abnormalities in Dravet Syndrome Mice Before and After the Age of Seizure Onset. Brain research, 1724, 146399.
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6

Rectal Temperature Measurement in Mice

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Body temperature was measured by means of rectal thermometry with the TCAT-2AC Animal Temperature Control (Physitemp, New Jersey, USA) equipped with RET-3 Rectal Probe for Mice.
Mignogna M.L., Musardo S., Ranieri G., Gelmini S., Espinosa P., Marra P., Belloli S., Murtaj V., Moresco R.M., Bellone C, & D’Adamo P. (2021). RAB39B-mediated trafficking of the GluA2-AMPAR subunit controls dendritic spine maturation and intellectual disability-related behaviour. Molecular Psychiatry, 26(11), 6531-6549.
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7

Evaluating 5-HT1A Receptor Sensitivity

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8-OH DPAT is a selective 5-HT1A receptor agonist causing hypothermia mediated by the stimulation of 5-HT1A autoreceptors (Goodwin et al., 1985) . To assess the sensitivity of 5-HT1A auto-receptors after repeated treatment with fluoxetine, Mecp2null, Mecp2 Het and WT mice received 0.2 mg/kg 8-OH DPAT subcutaneously (s.c).
This dose was chosen on the basis of a dose-response curve (data not shown) because it causes an intermediate reduction of body temperature that allow to detect either potentiation or reduction of 8-OH-DPAT effects. Core temperature was measured by a BAT-12 Microprobe Thermometer equipped with a RET-3 rectal probe for mice (Physitemp, USA), every 15 minutes over a 2 h period. Temperature measurements were taken between 8:00 and 12:00 A.M.
Villani C., Sacchetti G., Carli M., & Invernizzi R.W. (2020). Fluoxetine rescues rotarod motor deficits in Mecp2 heterozygous mouse model of Rett syndrome via brain serotonin.
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8

Protein Effects on Mouse Thermoregulation

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Wild-type (WT; C57BL/6J) and Glp1r -/-mice were fasted for 5 h (9:00-14:00).
Basal rectal temperature was measured using a thermocouple probe (RET-3 rectal probe for mice, Physitemp, Clifton, NJ, USA) connected to a microprobe thermometer (BAT-12, Physitemp). Following the oral administration of soy or whey protein solution (2 g/kg, 20 mL/kg) or saline (control, 20 mL/kg), rectal temperature was measured at 1, 2, and 3 h after administration. Rectal temperature was measured in conscious mice and normal housing conditions (22 ± 2°C).
Ochiai K., Muto A., Seok B.S., Doi Y., Iwasaki Y., Okamatsu-Ogura Y., Drucker D.J, & Hira T. (2023). Glucagon-Like Peptide-1 Is Involved in the Thermic Effects of Dietary Proteins in Male Rodents. Endocrinology, 164(6).
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9

Evaluating 5-HT1A Receptor Sensitivity

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8-OH DPAT is a selective 5-HT1A receptor agonist causing hypothermia mediated by the stimulation of 5-HT1A autoreceptors (Goodwin et al., 1985) . To assess the sensitivity of 5-HT1A auto-receptors after repeated treatment with fluoxetine, Mecp2null, Mecp2 Het and WT mice received 0.2 mg/kg 8-OH DPAT subcutaneously (s.c).
This dose was chosen on the basis of a dose-response curve (data not shown) because it causes an intermediate reduction of body temperature that allow to detect either potentiation or reduction of 8-OH-DPAT effects. Core temperature was measured by a BAT-12 Microprobe Thermometer equipped with a RET-3 rectal probe for mice (Physitemp, USA), every 15 minutes over a 2 h period. Temperature measurements were taken between 8:00 and 12:00 A.M.
Villani C., Sacchetti G., Carli M, & Invernizzi R.W. (2020). Fluoxetine rescues rotarod motor deficits in Mecp2 heterozygous mouse model of Rett syndrome via brain serotonin. Neuropharmacology, 176.
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