LC–MS grade, acetonitrile (MeCN), methanol (MeOH), tetrahydrofuran (THF), dimethyl sulfoxide, MS grade formic acid (HCOOH) and all the analytical standards of testosterone, dihydrotestosterone, α-estradiol, β-estradiol, methyltestosterone, as well as the hormone preparations used for in vitro measurements of platelet activation/reactivity were obtained from Sigma (St. Louis, MO, USA) and had a minimum purity specification of 99%. Nitric acid (HNO3) was purchased from POCH (Gliwice, Poland). Dichloromethane (DCHM, HPLC grade) was provided by VWR International (Radnor, PA, USA).
PBS was from Avantor Performance Materials Poland S.A. (Gliwice, Poland). Arachidonate, equine tendon collagen and ADP were from Chrono-Log Corp. (Havertown, PA, USA). Fluorolabelled monoclonal antibodies (moAbs): anti-CD61/PerCP, antiCD62/PE, PAC-1/FITC, isotype controls IgG/PE and IgM/FITC, as well as CellFix (1% formaldehyde in PBS) were from Becton Dickinson (San Diego, CA, USA). Ultrapure water was obtained from Milli-Q purification system (Millipore, Bedford, MA, USA). Nitrogen (NM32LA Nitrogen Generator, Peak Scientific Instruments, Billerica, MA, USA) was used as a drying gas.
PBS was from Avantor Performance Materials Poland S.A. (Gliwice, Poland). Arachidonate, equine tendon collagen and ADP were from Chrono-Log Corp. (Havertown, PA, USA). Fluorolabelled monoclonal antibodies (moAbs): anti-CD61/PerCP, antiCD62/PE, PAC-1/FITC, isotype controls IgG/PE and IgM/FITC, as well as CellFix (1% formaldehyde in PBS) were from Becton Dickinson (San Diego, CA, USA). Ultrapure water was obtained from Milli-Q purification system (Millipore, Bedford, MA, USA). Nitrogen (NM32LA Nitrogen Generator, Peak Scientific Instruments, Billerica, MA, USA) was used as a drying gas.