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Lna microrna ish mir 122 optimization kit

Manufactured by Qiagen
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The LNA™ microRNA ISH miR-122 optimization kit is a laboratory equipment product developed by Qiagen. The core function of this kit is to facilitate the in situ hybridization (ISH) of microRNA-122 (miR-122) using Locked Nucleic Acid (LNA) technology.

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Lab products found in correlation

Nuclear fast red, by Vector Laboratories (2 mentions) Sheep anti digoxigenin ap, by Roche (2 mentions) Nbt bcip, by Vector Laboratories (2 mentions) Nbp2 14833, by Novus Biologicals (2 mentions)

2 protocols using lna microrna ish mir 122 optimization kit

1

Immunohistochemistry and In Situ Hybridization Protocols

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
IHC was performed as previously described69 (link) using a 1:400 antibody dilution for PKM2 (C-11), 1:600 dilution for PKM1 (Cat# NBP2-14833, Novus Biologicals; Littleton, CO), and 1:250 dilution for GLUT1 (AbCam; Cat# ab652). ISH was performed as described70 (link) using LNA™ microRNA ISH miR-122 optimization kit (Cat# 90003, Exiqon; Woburn, MA) followed by incubation of sheep anti-digoxigenin-AP (Cat# 11093274910, Roche Diagnostics; Mannheim, Germany), and developed with NBT:BCIP (Cat# SK-5400, Vector Laboratories; Burlingame, CA) at 30°C overnight. Nuclear fast red was used to counterstain nuclei (Cat# H-3403, Vector Laboratories).
Fong M.Y., Zhou W., Liu L., Alontaga A.Y., Chandra M., Ashby J., Chow A., O’Connor S.T., Li S., Chin A.R., Somlo G., Palomares M., Li Z., Tremblay J.R., Tsuyada A., Sun G., Reid M.A., Wu X., Swiderski P., Ren X., Shi Y., Kong M., Zhong W., Chen Y, & Wang S.E. (2015). Breast cancer-secreted miR-122 reprograms glucose metabolism in pre-metastatic niche to promote metastasis. Nature cell biology, 17(2), 183-194.
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2

Immunohistochemistry and In Situ Hybridization Protocols

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
IHC was performed as previously described69 (link) using a 1:400 antibody dilution for PKM2 (C-11), 1:600 dilution for PKM1 (Cat# NBP2-14833, Novus Biologicals; Littleton, CO), and 1:250 dilution for GLUT1 (AbCam; Cat# ab652). ISH was performed as described70 (link) using LNA™ microRNA ISH miR-122 optimization kit (Cat# 90003, Exiqon; Woburn, MA) followed by incubation of sheep anti-digoxigenin-AP (Cat# 11093274910, Roche Diagnostics; Mannheim, Germany), and developed with NBT:BCIP (Cat# SK-5400, Vector Laboratories; Burlingame, CA) at 30°C overnight. Nuclear fast red was used to counterstain nuclei (Cat# H-3403, Vector Laboratories).
Fong M.Y., Zhou W., Liu L., Alontaga A.Y., Chandra M., Ashby J., Chow A., O’Connor S.T., Li S., Chin A.R., Somlo G., Palomares M., Li Z., Tremblay J.R., Tsuyada A., Sun G., Reid M.A., Wu X., Swiderski P., Ren X., Shi Y., Kong M., Zhong W., Chen Y, & Wang S.E. (2015). Breast cancer-secreted miR-122 reprograms glucose metabolism in pre-metastatic niche to promote metastasis. Nature cell biology, 17(2), 183-194.
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