Nih 3t3

CS (MW of 50–190 kDa, DA: 15–25%), DS (MW of 7–20 kDa), PEI (MW of 1.3 kDa), polysorbate 80, Leu, Mant, Mans, doxorubicin hydrochloride, polydimethylsiloxane (PDMS), o-phthaldialdehyde (OPA), 2-mercaptoethanol, acetonitrile, sodium bicarbonate, dimethyl sulfoxide (DMSO), fluorescein isothiocyanate isomer I (FITC), cellulose dialysis tubing with cutoff 12 kD MWCO, and benzoylated dialysis tubing with cutoff 2000 NMWCO were purchased from Sigma Chemical Co. (St. Louis, MO, USA). miRIDIAN microRNA Mimics for precursor hsa-mir-34a-5p was purchased from PerkinElmer. Non-small human adenocarcinoma epithelial cell line (A549) and mouse embryonic fibroblast cell line NIH/3T3 (ATCC CRL-1658TM) were obtained from the European Collection of Authenticated Cell Cultures (ECACC). Roswell Park Memorial Institute (RPMI) 1640 Medium, Dulbecco’s Modified Eagle Medium (DMEM), Trypsin-EDTA, and fetal bovine serum (FBS) were purchased from Biosera (France). Antibiotic–antimycotic and MTT were prepared from Biowest (USA) and Duchefa (Biochemie, Netherlands), respectively. All other chemicals used in the study were of analytical grade.

The cancer cell lines utilized for our investigations, including human ovarian carcinoma (A2780), human cervix carcinoma (Hela), human breast adenocarcinomas (MCF-7 and MDA-MB-231), and mouse embryonic fibroblast cell line (NIH/3T3) were purchased from the European Collection of Authenticated Cell Cultures (ECACC, Salisbury, UK). Another cervical cell line (SiHa) was acquired from the American Tissue Culture Collection (Manassas, VA, USA). For optimal growth, all cell lines were maintained in minimal essential medium (MEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% MEM non-essential amino acid solution (MEM-NEAA), and 1% penicillin/streptomycin mixture, at 37 °C, in a humidified incubator, containing 5 % carbon dioxide (CO₂). All the utilized media and supplements were purchased from Lonza Group Ltd. (Basel, Switzerland). Unless otherwise specified, the chemicals and kits used for the experiments were purchased from Merck Life Science Ltd. (Budapest, Hungary).