The assay was performed as previously described (59 (link)). Immulon 4HB ultrahigh binding microtiter plates (Thermo Scientific) were coated with 0.5 mg/mL protein A in coating buffer at 4°C overnight. Wells were washed three times and blocked with assay buffer. Human CD33 rSiglec-Fc chimeras (R & D Systems) were added to wells at 0.5 mg/mL and adhered at 4°C overnight. Wells were washed three times with assay buffer. 107 FITC-labeled bacteria suspended in 50 μL assay buffer were added into the well and incubated at 37°C for 10 min. After washing, the fluorescent intensity (excitation 488 nm, emission 530 nm) was measured using a SpectraMax Paradigm Multi-Mode Detection Platform (Molecular Devices).
Immulon 4hb ultrahigh binding microtiter plates
Manufactured by Thermo Fisher Scientific
Immulon 4HB ultrahigh binding microtiter plates are laboratory equipment designed for use in various immunoassay applications. These plates feature a high-binding surface that enables efficient immobilization of biomolecules, such as proteins, antibodies, or antigens, for subsequent analysis or detection.
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2 protocols using immulon 4hb ultrahigh binding microtiter plates
1
Bacterial Binding Assay with CD33-Fc
2
Bacterial Binding Assay with CD33-Fc
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The assay was performed as previously described (59 (link)). Immulon 4HB ultrahigh binding microtiter plates (Thermo Scientific) were coated with 0.5 mg/mL protein A in coating buffer at 4°C overnight. Wells were washed three times and blocked with assay buffer. Human CD33 rSiglec-Fc chimeras (R & D Systems) were added to wells at 0.5 mg/mL and adhered at 4°C overnight. Wells were washed three times with assay buffer. 107 FITC-labeled bacteria suspended in 50 μL assay buffer were added into the well and incubated at 37°C for 10 min. After washing, the fluorescent intensity (excitation 488 nm, emission 530 nm) was measured using a SpectraMax Paradigm Multi-Mode Detection Platform (Molecular Devices).
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