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Cytotell ultragreen

Manufactured by AAT Bioquest
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CytoTell UltraGreen is a fluorescent dye that can be used to stain cellular nucleic acids. It has an excitation maximum at 509 nm and an emission maximum at 525 nm, allowing it to be used with common fluorescence detection equipment.

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Lab products found in correlation

Anti cd25 clone pc61, by BioLegend (1 mentions) Zombie violet fixable viability kit, by BioLegend (1 mentions) Anti ifn γ, by BD (1 mentions) Anti mouse cd28, by BD (1 mentions) Cytotell red 650, by AAT Bioquest (1 mentions) Anti mouse secondary igg hrp linked f ab 2 fragment clone na9310v, by Cytiva (1 mentions) Anti t bet, by BioLegend (1 mentions) Anti foxp3 clone mf 14, by BioLegend (1 mentions) Anti cd4 fitc, by BD (1 mentions)

2 protocols using cytotell ultragreen

1

Antibody Panel for T Cell Analysis

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Antibodies used in this study: anti-hamster IgG, whole molecule (Sigma-Aldrich, St. Louis, MO), NA/LE hamster anti-mouse CD3ε, anti-mouse CD28 (BD Biosciences), anti-PRMT5 (A-11, Santa Cruz Biotechnology, Dallas, Tx), anti-H3R2me2s polyclonal (Invitrogen, Waltham, MA), alpha-tubulin mouse (DM1A, Cell Signaling Technologies (CST), Danvers, MA), anti-mouse secondary-IgG HRP linked F(ab’)2 fragment (clone NA9310V Amersham Biosciences, Piscataway, NJ), anti-rabbit secondary – IgG HRP linked whole antibody (clone NA934V, Amersham Biosciences), anti-PRMT5 rabbit monoclonal (clone ST51-06, Invitrogen), anti-H3R2me2s ChIP-seq grade (EpigenTek, Farmingdale, NY), anti-CD4 FITC (Clone H129.19, BD Biosciences), anti-CD25 (clone PC61, BioLegend, San Diego, CA), anti-Foxp3 (clone MF-14, BioLegend), anti-IFNγ (BD Biosciences), anti-Tbet (BioLegend), nuclear stain Draq5 (ThermoFisher Scientific), anti-rabbit IgG Fab2 Alexafluor 488 (CST), zombie violet fixable viability kit (BioLegend), CytoTell UltraGreen and CytoTell Red650 (AAT Bioquest, Pleasanton, CA).
Jadon N., Shanthalingam S., Tew G.N, & Minter L.M. (2024). PRMT5 regulates epigenetic changes in suppressive Th1-like iTregs in response to IL-12 treatment. Frontiers in Immunology, 14, 1292049.
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2

CytoTell UltraGreen Cell Proliferation Assay

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The cell proliferation assay was performed using CytoTell™ UltraGreen (AAT Bioquest) according to the manufacturer's protocol.
He X., Kläsener K., Iype J.M., Becker M., Maity P.C., Cavallari M., Nielsen P.J., Yang J, & Reth M. (2018). Continuous signaling of CD79b and CD19 is required for the fitness of Burkitt lymphoma B cells. The EMBO Journal, 37(11), e97980.
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