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Goat anti mouse second antibody

Manufactured by Cell Signaling Technology
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Goat anti-mouse second antibody is a secondary antibody used in immunoassays and other experimental techniques. It is produced in goats and specifically binds to mouse primary antibodies, allowing for the detection and quantification of target proteins.

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Lab products found in correlation

Goat anti rabbit second antibody, by Cell Signaling Technology (2 mentions) Ab8226, by Abcam (1 mentions) Ep38y, by Abcam (1 mentions) Ab206423, by Abcam (1 mentions) Vimentin, by Abcam (1 mentions) E cadherin, by Abcam (1 mentions) N cadherin, by Abcam (1 mentions) C myc, by Abcam (1 mentions) β actin, by Abcam (1 mentions) β catenin, by Abcam (1 mentions) α catenin, by Abcam (1 mentions) Wnt2b, by Abcam (1 mentions) Ab32072, by Abcam (1 mentions)

Close spelling variants of this product

Second antibody (24 citations)

2 protocols using goat anti mouse second antibody

1

Validated Protein Expression Quantification

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The following antibodies were used for western blot: MYL1 (Abcam, ab151749, 1:1000), EGF (Abcam, ab206423, 1:1000), EGFR (Abcam, EP38Y, 1:1000), β-actin (Abcam, ab8226, 1:1000), Goat anti-mouse second antibody (Cell Signaling Technology, 5470 S, 1:15000), Goat anti-rabbit second antibody (Cell Signaling Technology, 5151 S, 1:30000). PENTER control plasmid (Shandong Vigene Biosciences, Jinan, China) and pENTER-MYL1 plasmid (Shandong Vigene Biosciences, Jinan, China) were used for transfection.
Li C., Guan R., Li W., Wei D., Cao S., Chang F., Wei Q., Wei R., Chen L., Xu C., Wu K, & Lei D. (2023). Analysis of myosin genes in HNSCC and identify MYL1 as a specific poor prognostic biomarker, promotes tumor metastasis and correlates with tumor immune infiltration in HNSCC. BMC Cancer, 23, 840.
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2

Western Blot Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for western blot: c-Myc (Abcam, ab32072, 1:1000), Wnt2b (Abcam, ab178418, 1:1000), beta Catenin (Abcam, ab68183, 1:1000), E-cadherin (Abcam, ab40772, 1:1000), α-catenin (Abcam, ab51032, 1:1000), N-cadherin (Abcam, ab76011, 1:1000), Vimentin (Abcam, ab92547, 1:1000), β-actin (Abcam, ab8226, 1:1000), Goat anti mouse second antibody (Cell Signaling Technology, 5470S, 1:15000), Goat anti rabbit second antibody (Cell Signaling Technology, 5151S, 1:30000).
Li C., Li W., Cao S., Xu J., Qian Y., Pan X., Lei D, & Wei D. (2021). Circ_0058106 promotes proliferation, metastasis and EMT process by regulating Wnt2b/β-catenin/c-Myc pathway through miR-185-3p in hypopharyngeal squamous cell carcinoma. Cell Death & Disease, 12(11), 1063.
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