Rabbit anti sox2

Cells were fixed with 4% paraformaldehyde for 20 min at room temperature (RT) and blocked in a solution of Mg²⁺ and Ca²⁺ free PBS containing 5% horse serum and 0.3% Triton-X for 1 h at room temperature and followed by incubation with primary antibody at 4°C overnight. The following primary antibodies and dilutions have been used in this study: mouse anti-Flavivirus group antigen antibody (ZIKV E) (1:2000; Millipore, clone D1-4G2-4-15), mouse anti-Ki67 (1:200, DAKO, MIB-1), rabbit anti-Ki67 (1:500, Thermofisher, SP-6), rabbit anti-cleaved caspase-3 (1:1000, Cell Signaling Technology, Asp15), goat anti-SOX2 (1:100, Santa Cruz, sc-17320), rabbit anti-SOX2 (1:200, Biolegend, N-term), mouse anti-NESTIN (1:1000, Neuromics, MO15012), rabbit anti-TUJ1 antibody (1:500, Covance, MRB-435P), and chicken anti-MAP2 (1:1000, Abcam, Ab5392). The secondary antibodies include donkey anti-mouse, goat, rabbit or chicken secondary antibodies conjugated with Alexa-Fluor-488, Alexa-Fluor-594 or Alexa-Fluor-647 fluorophore (1:500, Life Technologies). Nuclei were counterstained by DAPI.
The immunohistochemical analysis of mouse tissues was performed on cryosections. In brief, the tissues were fixed overnight with 4% PFA at 4°C, sunk in 20% sucrose, embedded in OCT, and then sectioned at 30 μm for immunostaining. The primary and secondary antibodies were used as described above.