Anti jnk

LPS, PLA, and SP600125 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dimethyl sulfoxide, which was also purchased from Sigma-Aldrich, was used as a solvent for SP600125. Fetal bovine serum was purchased from Life Technologies Corporation (Carlsbad, CA, USA). Anti-JNK was purchased from Zhong Shan-Golden Bridge (Beijing, China). Anti-phospho-JNK and anti-β-actin were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Chemiluminescence reagents were purchased from Thermo Scientific (Rockford, IL, USA). ELISA kits for the detection of IL-6 and IL-8 were purchased from Senxiong (Shanghai, China).

DMEM high sugar medium was purchased from Hyclone Corporation (USA); FBS was obtained from Gibco Corporation (USA); ALP staining kit and substrate, vitamin C, β-phosphoglycerol, and Alizarin Red S were ordered from Sigma Corporation (USA); Anti-β-actin antibodies was obtained from Biyuntian Company (Jiangsu, China); Anti-Runx2, anti-OPN, anti-OCN antibodies, and the secondary antibodies were ordered from Cell Signaling Technology (CST, USA); Anti-Smad1/5/8, anti-p38, anti-Erk1/2, and anti-JNK antibodies were obtained from Zhongshan Golden Bridge Biotechnology (Beijing, China); Anti- phospho-Smad1/5/8, anti-phospho-p38, anti-phospho-Erk1/2, and anti-phospho-JNK antibodies were ordered from Santa Cruz Corporation (USA). The recombinant adenoviruses including Ad-CCN3, Ad-siCCN3, Ad-GFP, Ad-RFP, Ad-BMP9, Ad-DLL1, and Ad-Hey1 were kindly provided by Dr Tong-Chuan He (University of Chicago Medical Center, Chicago, IL, USA). GFP and RFP were used as tags for tracking the viruses, and Ad-GFP and Ad-RFP were used as vector controls. The effectiveness of the above viruses was confirmed in our and Professor He’s previous experiments^{30 (link)}.