Normal human lung fibroblasts (NHLFs, CRL1490 cells from ATCC, Manassas, VA, USA) were cultured in fibroblast basal medium supplemented with 2% fetal bovine serum (FBS), 0.1% recombinant human fibroblast growth factor basic (rhFGF-B), 0.1% insulin, and 0.1% gentamicin/amphotecin-B (Lonza, Walkersville, MD, USA), and used in passages 3–6.
NHFLs were starved in the serum-free medium for 18 h to induce cell cycle synchronization,49 (link) and then refreshed with complete culture medium. The cells were seeded on the substrates at a density of 4000 cells/cm2 and cultured at 37 °C and 5% CO2 for a predefined period of time before the analyses.
NHFLs were starved in the serum-free medium for 18 h to induce cell cycle synchronization,49 (link) and then refreshed with complete culture medium. The cells were seeded on the substrates at a density of 4000 cells/cm2 and cultured at 37 °C and 5% CO2 for a predefined period of time before the analyses.