A total of 1 million passage 3 cells were resuspended in 200 µL of PBS containing fluorescein isothiocyanate– or phycoerythrin-coupled antibodies against CD34, CD44, CD45, CD90, CD147, CD271 (BD Biosciences), CD117 (eBioscience), CD105, CD166 (Ancell), and vascular endothelial growth factor receptor–2 (VEGFR-2; R&D Systems) and incubated for 30 minutes at 4°C. The cells were then washed with PBS and resuspended in 350 µL of PBS for analysis. Fluorescein isothiocyanate– or phycoerythrin-coupled nonspecific mouse Immunoglobulin G (BD Biosciences) was used as an isotype control. Cell fluorescence was evaluated via flow cytometry using an EC800 instrument (SONY), and data were also analyzed using the EC800 instrument.9 (link),23 (link),24 (link),32 (link)
Ec800 instrument
Manufactured by Sony
Sourced in Japan
The EC800 instrument is a precision laboratory equipment used for electrochemical analysis. It measures and records electrochemical properties of materials and solutions. The core function of the EC800 is to perform accurate and reliable electrochemical measurements for research and testing purposes.
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Lab products found in correlation
2 protocols using ec800 instrument
1
Flow Cytometric Characterization of Stem Cells
2
Comprehensive Immunophenotypic Analysis of Cells
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One million cells at passage 2 were resuspended in 200 mL of phosphate-buffered saline solution (PBS) containing antibodies coupled with either fluorescein isothiocyanate or phycoerythrin. Coupled antibodies to cluster of differentiation (CD)31 (endothelial cell marker), CD34 (hematopoietic progenitor cell antigen), CD44 (hyaluronan receptor), CD45 (hematopoietic cell marker), CD90 (Thy1), CD147 (neuregulin; BD Bioscience, San Diego, CA), CD117 (C-kit, stem cell factor receptor; eBioscience, San Diego, CA), CD105 (SH-2) and CD166 (ALCAM, SB10; Ancell, Bayport, MN) were used. The vascular endothelial growth factor receptor 2 (VEGFR2) was obtained from R&D Systems (Minneapolis, MN). After incubation for 30 minutes at 4 C, the cells were washed with PBS and then resuspended in 350 mL of PBS for analysis. As isotype control for the primary antibody, fluorescein isothiocyanatee or phycoerythrin-coupled nonspecific mouse IgG (BS Biosciences) was used. Cell fluorescence measurements through flow cytometry and data analysis were carried out using an EC 800 instrument (SONY, Tokyo, Japan). 9, 10
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