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Phycoerythrin pe labeled anti human pd l1 monoclonal antibody

Manufactured by BioLegend
Sourced in United States

Phycoerythrin (PE)-labeled anti-human PD-L1 monoclonal antibody is a lab equipment product that can be used for the detection and analysis of PD-L1 expression on human cells. The antibody is conjugated with the fluorescent dye phycoerythrin, which allows for the visualization and quantification of PD-L1 positive cells through flow cytometry or other fluorescence-based techniques.

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2 protocols using phycoerythrin pe labeled anti human pd l1 monoclonal antibody

1

Importin-beta Knockdown Modulates Apoptosis

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IPZ, propidium iodide (PI), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-caspase-8 antibody (#9746), caspase-9 antibody (#9502), PUMA antibody (#12450), β-actin antibody (#4967), anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (#7074), and anti-mouse IgG HRP-linked antibody (#7076) were purchased from Cell Signaling Technology Japan, K.K. (Tokyo, Japan). The anti-importin beta mouse monoclonal antibody [3E9] (ab2811) was purchased from abcam (Cambridge, UK). The phycoerythrin (PE)-labeled anti-human PD-L1 monoclonal antibody (#329706), PE-conjugated anti-mouse IgG2b antibody (#400314), and anti-p63(ΔN) antibody (#619001) were purchased from BioLegend (San Diego, CA, USA). Ambion Silencer® Select Pre-designed siRNA against the gene-encoding KPNB1 (cat. no. s7919) and Silencer® Select Negative #1 Control siRNA (cat. no. AM4611) were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA).
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2

Regulation of PD-L1 by KPNB1 and IRF1

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The KPNB1 inhibitor importazole (IPZ) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). β-actin antibody (#4967), GAPDH (D16H11) XP® rabbit monoclonal antibody (#5174), IRF1 (D5E4) XP® rabbit monoclonal antibody (#8478), KPNA2 rabbit antibody (#14372), and anti-rabbit IgG horseradish peroxidase (HRP)-conjugated antibody (#7074) were purchased from Cell Signaling Technology Japan, K.K. (Tokyo, Japan). Phycoerythrin (PE)-labeled anti-human PD-L1 monoclonal antibody (#329706) and PE-conjugated anti-mouse IgG2b antibody (#400314) were purchased from BioLegend (San Diego, CA, USA). Ambion Silencer® Select Pre-designed small interfering RNA (siRNA) against the genes encoding IRF1 (cat. no. s7501) and KPNA2 (cat. no. s7922), and Silencer® Select Negative #1 Control siRNA (cat. no. AM4611) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
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