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2 protocols using goat anti ddr2

1

Collagen I Immunofluorescence and Western Blotting

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Collagen I (acid-soluble from rat tail) was from Sigma (Gillingham, UK). The antibodies and their sources were as follows: antibodies for immunofluorescence: mouse anti-HA-tag monoclonal antibody (dilution 1:200; Cell Signaling Technology), rabbit anti-calnexin polyclonal antibody (dilution 1:200, Santa Cruz), Alexa Fluor 568-goat anti-mouse IgG (dilution 1:200; Molecular Probes), Alexa Fluor 568-goat anti-rabbit IgG (dilution 1:200; Molecular Probes), Alexa Fluor 488-goat anti mouse IgG (dilution 1:200, Molecular Probes). Antibodies for Western blotting: goat anti-DDR2 from R & D Systems (Abingdon, UK); mouse anti-phosphotyrosine, clone 4G10, from Upstate Biotechnology (Lake Placid, NY); sheep anti-mouse Ig-horseradish peroxidase (Amersham Biosciences UK, Chalfont St Giles, UK); rabbit anti-goat Ig-horseradish peroxidase (Zymed Laboratories, San Francisco, CA).
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2

Osteogenic Differentiation Assay

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Goat anti-DDR2 (R&D Systems, MN, USA), rabbit anti-Nrp1, mouse anti-β-actin (Abcam, MA, USA), goat anti-Runx2 (Santa Cruz, TEX, USA), rabbit anti-ERK1/2 (Cell Signaling Technology, MA, USA). BMP-2 (Peprotech, NJ, USA), Ascorbic acid (AA), β-glycerophosphate (β-GP) (Sigma, IL, USA).
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