De maldi tof spectrometer

All reagents used were purchased from Sigma Aldrich (St. Louis, MO) unless otherwise stated. G3-PAMAM succinamic acid (10 wt.%) in methanol was obtained from Dendritech (Midland, MI) and it was dried in vacuo prior to use. 4-Dimethylaminopyridine (99%) Dimethylsulfoxide anhydrous (≥99.9%), N,N'-dicyclohexylcarbodiimide (99%) and Sodium bicarbonate (100%) were purchased from Sigma Aldrich and were used without further purifications. Sodium hydroxide (100%) and 2-Methylbutane (95%) were purchased from Fisher Scientific and were also used without further purification. Dendrimeric curcumin conjugates were purified by repeated ultrafiltration with deionized water using appropriate molecular weight cut-offs (Millipore’s Amicon Ultra centrifugal filters). ¹H- NMR spectra were obtained using an Agilent 600 NMR spectrophotometer operating at 599.77 MHz. Deuterated DMSO(DMSO-d₆) and Deuterated D₂O were used as NMR solvents and Chemical shifts (δ) are given in ppm with reference to the internal standard Tetramethylsilane (TMS, δ = 0 ppm). The molecular weights of the conjugates were analyzed using a Micromass M@LDI-R or Applied Biosystems DE Maldi-TOF spectrometer.