A series of 15 benzenesulfonamide derivatives (A1 –A15 ) were prepared as described [20 (link)]. For all experiments, a stock solution of each test compound was prepared in DMSO at 50 mM concentration and serially diluted to the desired concentrations in the respective culture media.
Dulbecco’s modified Eagle medium (DMEM), Roswell Park Memorial Institute (RPMI), and fetal bovine serum (FBS) were purchased from Corning, Inc. (Corning, NY, USA). DMSO and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were bought from Sigma-Aldrich (St. Louis, MO, USA). SLC-0111 was obtained from MedChemExpress (Monmouth Junction, NJ, USA). Anti-CA I (1:1000, cat#ab267475) antibody was purchased from Abcam (Cambridge, MA, USA), and antibodies specifically recognizing CA II (1:1000, cat#8612), CA IX (1:1000, cat#5649), and CA XII (1:1000, cat#5865) were from Cell Signaling Technology (Danvers, MA, USA). CA II, CA IX, and CA XII siRNA and control siRNA were provided by Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).
Dulbecco’s modified Eagle medium (DMEM), Roswell Park Memorial Institute (RPMI), and fetal bovine serum (FBS) were purchased from Corning, Inc. (Corning, NY, USA). DMSO and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were bought from Sigma-Aldrich (St. Louis, MO, USA). SLC-0111 was obtained from MedChemExpress (Monmouth Junction, NJ, USA). Anti-CA I (1:1000, cat#ab267475) antibody was purchased from Abcam (Cambridge, MA, USA), and antibodies specifically recognizing CA II (1:1000, cat#8612), CA IX (1:1000, cat#5649), and CA XII (1:1000, cat#5865) were from Cell Signaling Technology (Danvers, MA, USA). CA II, CA IX, and CA XII siRNA and control siRNA were provided by Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).