Antifungal activities were determined by a conidial germination assay using 48-well culture plates.34 (link) Conidia of P. litchii and F. verticillioides were collected from 7- and 3-day-old cultures of fungi growing on PDA, respectively. Conidia were diluted with half strength potato dextrose broth (PDB:water = 1:1), counted and immediately used in the bioassay. Test samples were dissolved and diluted with DMSO to yield a two-fold dilution series, and 10 μL of the sample solution was added to each well containing 490 μL arthroconidium suspensions to obtain the final concentration from 0.625 to 80 μg/mL. After 8 h incubation at 30°C on a rotary shaker at 150 rpm, both germinated and non-germinated conidia were counted under an optical microscope to calculate the spore germination percentages. A spore was considered to be germinated when the germ tube length was 1.5 times the spore diameter. Three duplicates were performed for each concentration and more than 200 conidia in each duplicate were counted. Thiram (tetramethylthiuram disulfide) was used as the positive control, and DMSO was used as the negative control. Inhibition rates and IC50 values were calculated by nonlinear regression analysis of logistic dose–respond curves (SPSS 16.0 statistic software).
Spss 16.0 statistic software
Manufactured by IBM
Sourced in United States
SPSS 16.0 is a statistical software package developed by IBM. It is designed to perform a wide range of data analysis and statistical procedures. The core function of SPSS 16.0 is to provide users with the tools to manage, analyze, and present data effectively.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using spss 16.0 statistic software
1
Antifungal Activity Assay of Compounds
2
Osteoarthritis Biomarker Evaluation
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All values are shown as mean ± standard deviation (SD). All statistical tests were conducted using the SPSS 16.0 statistic software (SPSS Inc., IL, USA). Multiple pairwise comparisons were adjusted using Bonferroni correction. Kruskal-Wallis ANOVA was employed to determine differences in OARSI scores and the concentrations of sGAG, CTXII, TNF-α and IL-1β, in synovial fluid lavage across treatment groups. The MRI variables were examined by two-way ANOVA with repeated measures to examine intergroup variability over time and between treatment and control groups. Other data were analyzed with one-way ANOVA followed by Tukey's multiple comparison tests (t-test). P-values <0.05 (two-tailed) were deemed statistically significant.
3
Cell Proliferation and ROR1 Expression
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Data are presented as the mean ± S.E.M for the indicated numbers of independently performed experiments. A two-tailed Student t-test was used to determine statistical differences in levels of cell proliferation. Mann-Whitney U test was used to evaluate the ROR1 expression in different gender. All analyses were performed using GraphPad Prism version 6 (GraphPad Software Inc, San Diego, CA, United States) and SPSS 16.0 statistic software (SPSS Inc, Chicago, IL). Differences were considered to be statistically significant when p < 0.05.
4
Statistical Analysis of Survival Rates
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SPSS 16.0 statistic software (SPSS Inc., Chicago, IL, United States) was used to analyze all data. All survival rate was analyzed by the Kaplan-Meier method using a long-rank test. Student’s t-test was employed to compare the differences between the two groups. The Χ2 was used to analyze the relationship between GRWD1 expression and clinical features. The value of P < 0.05 was considered statistically significant.
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