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Polyvinylpyrrolidoneiodine

Manufactured by Merck Group

Polyvinylpyrrolidone-iodine (PVP-I) is a complex compound consisting of polyvinylpyrrolidone and iodine. It is a broad-spectrum antimicrobial agent used for disinfection and antiseptic purposes.

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2 protocols using polyvinylpyrrolidoneiodine

1

Germ-Free Zebrafish Embryo Preparation

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GF zebrafish were prepared following established protocols31 (link)with some modifications. Briefly, embryos 6 h post-fertilization (hpf) were soaked in 0.1% polyvinylpyrrolidoneiodine (Sigma) for 2 min and washed 3 times in gnotobiotic zebrafish media (GZM). Thereafter, the embryos were further soaked in 0.003% bleach for 10 min and washed by GZM. Lastly, GF embryos were transferred to a 25-cm2 cell culture flask (Nest Biotechnology Co.) containing 30 mL of sterile GZM.
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2

Synthesis of Modified κ-Carrageenan Hydrogel

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Semi refined κ-carrageenan with molecular weight of 275 kDa was obtained from CV. Karagen Indonesia, Semarang, Central of Java, Indonesia. κcarrageenan contains 36.5% of 3,6-anhydrous-Dgalactose and 15.1% of sulfate. Acrylamide (≥99%) that used in this research was supplied from Merck (Cat. No: 800830) and Glyoxal (40% solution in water) was obtained from Merck, (Cat. No. 820610). Poly(vinylpyrrolidone)-Iodine from Sigma Aldrich (CAS No. 25655-41-8) is used as the active ingredient trapped in the modified SAP k-carrageenan material.
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