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2 protocols using 9 aminoanthracene

1

Cytotoxicity Assay and Mutagenicity Test

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Ham⿿s F-12 medium, heat inactivated fetal bovine serum (FBS), l-Glutamine, Penicillin and Streptomycin were obtained from Biological Industries Israel Beit-Haemek Ltd., Israel. Mitomycin C, benzo(a) pyrene, 2- Nitrofluorene, sodium azide, 9-aminoanthracene, histidine, biotin, Giemsa stain, acridine orange, cyclophosphamide and Methylthiazolyldiphenyl-tetrazolium bromide (MTT) were purchased from Sigma⿿Aldrich, St. Louis, MO. Colcemid was obtained from Life Technologies, Carlsbad, CA.
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2

Fabrication of Anthracene-based Materials

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Kynar-761 PVDF was provided by Arkema. Lexan 143R PC was acquired from Sabic. Chloroform and dimethylformamide (DMF) were obtained from different viable sources. MWCNTs (diameter 9.5 nm, length 1.5 μm) were acquired from Nanocyl (NC 7000). Anthracene, 9-Anthracene carboxaldehyde and 9-aminoAnthracene were procured from Sigma Aldrich.
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