MOTS-c was synthesized by a standard Fmoc-based solid phase synthesis methodology as described in our previous report [26] (link). All amino acids and coupling reagents were purchased from GL Biochem Ltd. (Shanghai, China). Compound C was purchased from MedChemExpress (Shanghai, China), dissolved in 10% DMSO, kept at -20°C, and diluted in corn oil immediately before i.p. injection (10 mg/kg). Lipopolysaccharide (LPS) was purchased from Sigma (St Louis, USA) and dissolved in saline (1 μg/μL). Morphine hydrochloride was obtained from Shenyang First Pharmaceutical Factory (Shenyang, China). Celebrex was obtained from Medchemexpress (Cat No. 169590-42-5).
Coupling reagents
Manufactured by GL Biochem
Sourced in China
Coupling reagents are chemical compounds used to facilitate the formation of chemical bonds between molecules. They are commonly utilized in various laboratory applications, including peptide synthesis, bioconjugation, and organic synthesis. Coupling reagents help activate functional groups, enabling the formation of new covalent linkages between the target molecules.
Automatically generated - may contain errors
Lab products found in correlation
Compound c, by MedChemExpress (1 mentions)
Lipopolysaccharide lps, by Merck Group (1 mentions)
Agilent 1260 infinity, by Agilent Technologies (1 mentions)
5 bromo 4 chloro 3 indolylphosphate, by Thermo Fisher Scientific (1 mentions)
Ni sepharose 6 fast flow beads, by GE Healthcare (1 mentions)
Dipeptides, by Merck Group (1 mentions)
Amino acids, by GL Biochem (1 mentions)
Dynabeads myone streptavidin c1, by Thermo Fisher Scientific (1 mentions)
Smz745t microscope, by Nikon (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Lysozyme, by Merck Group (1 mentions)
Ultraflextreme maldi tof tof mass spectrometer, by Bruker (1 mentions)
Microflex maldi tof mass spectrometer, by Bruker (1 mentions)
2 protocols using coupling reagents
1
Synthesis and Application of MOTS-c
2
Peptide-based Protein Purification Protocol
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General chemicals and reagents were purchased from Fisher Acros (Hampton, NH, USA). Isothiocyanobenzyl-NTA was purchased from Biomass (Rockville, MD, USA). Tentagel S NH2 resin was purchased from RAPP polymer (Tuebingen, Germany). DynabeadsTM MyOneTM Streptavidin C1, Streptavidin-alkaline phosphatase (SAAP), and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) were obtained from Thermofisher (Hampton, NH, USA). Amino acids, coupling reagents, and biotin were purchased from GL-Biochem. BSA (Shanghai, China), lysozyme, and dipeptides were obtained from Sigma-Aldrich (St. Louis, MO, USA). Ni Sepharose 6 FastFlow beads were obtained from GE Healthcare (Chicago, IL, USA).
MALDI-TOF-MS and MALDI-TOF-MS/MS analysis were performed by a Bruker Micro-flex MALDI-TOF mass spectrometer (Billerica, MA, USA) and a Bruker Ultra-flextreme MALDI-TOF/TOF mass spectrometer. The HPLC analysis and HPLC purification were performed using Agilent 1260 Infinity (Agilent, Santa Clara, CA, USA). Positive bead (blue beads) screening was performed with a Nikon SMZ 745T microscope (Nikon, Tokyo, Japan).
MALDI-TOF-MS and MALDI-TOF-MS/MS analysis were performed by a Bruker Micro-flex MALDI-TOF mass spectrometer (Billerica, MA, USA) and a Bruker Ultra-flextreme MALDI-TOF/TOF mass spectrometer. The HPLC analysis and HPLC purification were performed using Agilent 1260 Infinity (Agilent, Santa Clara, CA, USA). Positive bead (blue beads) screening was performed with a Nikon SMZ 745T microscope (Nikon, Tokyo, Japan).
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