Amine-modified fluorescent latex beads (Sigma) were washed twice with 400 μL of PBS and centrifuged at 3000 g for 3 min.33 400 μL of 8% glutaraldehyde (diluted in PBS) was added to the beads and incubated on a roller overnight at 4°C. After washing with PBS, 1mg/ml of recombinant MSP2 was added to the mixture and incubated while vortexing for 4 hours. Following this, the mixture was centrifuged, and the pellet was collected as the bound protein fraction. 200ul of ethanolamine was added to the pellet to quench amine groups and incubated for 30 min while vortexing. The pellet was subsequently washed in PBS and blocked with 1% BSA overnight at 4°C. The antigen-coated beads were stored 4°C in the presence of 0.1% SDS and 0.02% sodium azide.
Amine modified fluorescent latex beads
Manufactured by Merck Group
Amine-modified fluorescent latex beads are a type of laboratory equipment used for various applications. These beads are made of fluorescent latex and have amine functional groups on their surface, which can be used for covalent coupling or adsorption of biomolecules. The core function of these beads is to serve as a versatile platform for labeling, detection, and analysis in research and diagnostic applications.
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Lab products found in correlation
3 protocols using amine modified fluorescent latex beads
1
Amine-Modified Fluorescent Latex Bead Assay
2
Coating Fluorescent Beads with Recombinant CSP
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Amine-modified fluorescent latex beads of 2.0-µm size (Sigma-Aldrich) were washed twice with 400 µl of PBS and centrifuged at 2000 × g for 3 min. Glutaraldehyde of 8% in PBS was added to the beads and incubated on a roller overnight at 4 °C. After washing with PBS, 1 mg/ml of recombinant CSP was added to the beads and incubated on a vortex for 6 h. Subsequently, the beads were centrifuged and resuspended in 200 µl of ethanolamine and incubated for 30 min on a vortex to quench all the remaining amine groups. The beads were subsequently washed in PBS and blocked with 1% BSA overnight at 4 °C. The antigen-coated beads were kept in a sonicating water bath for 20 min at 4 °C to reduce aggregation and subsequently adjusted to 5 × 107 beads/ml. The beads were stored at 4 °C in the presence of 0.1% SDS and 0.02% NaN3.
3
Amine-Modified Fluorescent Latex Bead Coupling
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Amine-modified fluorescent latex beads (Sigma) were washed twice with 400ul of PBS and centrifuged at 3000g for 3 min (76). 400uL of 8% glutaraldehyde (diluted in PBS)
was added to the beads and incubated on a roller overnight at 4°C. After washing with PBS, 1mg/ml of recombinant MSP2 was added to the mixture and incubated on a vortex for 4 hours. Subsequently, the mixture was centrifuged, and the pellet was collected as the bound protein fraction. 200ul of ethanolamine was added to the pellet to quench amine groups and incubated for 30 min on the vortex. The pellet was subsequently washed in PBS and blocked with 1% BSA overnight at 4°C. The antigencoated beads were stored 4°C in the presence of 0.1% SDS and 0.02% sodium azide.
was added to the beads and incubated on a roller overnight at 4°C. After washing with PBS, 1mg/ml of recombinant MSP2 was added to the mixture and incubated on a vortex for 4 hours. Subsequently, the mixture was centrifuged, and the pellet was collected as the bound protein fraction. 200ul of ethanolamine was added to the pellet to quench amine groups and incubated for 30 min on the vortex. The pellet was subsequently washed in PBS and blocked with 1% BSA overnight at 4°C. The antigencoated beads were stored 4°C in the presence of 0.1% SDS and 0.02% sodium azide.
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