Thiazolyl blue tetrazolium bromide mtt reagent kit

Ambroxol (ABX), kappa-carrageenan (CRG_K), sodium alginate (SA), and chitosan (CS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Adenocarcinomic human alveolar basal epithelial cell (A549) lung cancer cells were purchased from ATCC (Manassas, VA, USA). The A549 human lung cancer cells were cultured in DMEM (Giboco, UK) supplied with foetal bovine serum and penicillin/streptomycin. The cell line was grown at 37 °C under a humidified atmosphere with 5% CO2 to 80% to 90% confluence. The Thiazolyl Blue Tetrazolium Bromide (MTT) reagent kit was purchased from ABCAM, Cambridge, UK. Other chemicals used were of analytical grade. The Annexin V-FITC Apoptosis Detection Kit and Cell cycle kits were purchased from BD Pharmingen (San Diego, CA, USA). Caspase-3, nuclear factor kappa-B (NF-kB), interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumour necrosis factor-alpha (TNF-α) ELISA kits were procured from Invitrogen^®, Thermo Fisher Scientific, Waltham, CA, USA.

Naringenin, isopropyl myristate, castor oil, and olive oil were purchased from Sigma-Aldrich, (St. Louis, MO, USA). Ethanol, Tween 20, Tween 80, and PEG 200 were purchased from Merck, USA. Sefsol 218 was procured from Nikko Chemicals (Tokyo, Japan), whereas Capryol 90, Transcutol HP, and Labrafac PG were obtained from Gattefosse (Saint Priest Cedex, France). The Caspase-3 Colorimetric Assay Kit (Catalog No. K106) was purchased from BioVision, USA. Human Bax ELISA (EIA-4487) was purchased from DRG International, Inc., USA. The Bcl-2 ELISA Kit (Cat. No. 99-0042) was procured from Invitrogen Corporation, CA, USA. The Thiazolyl Blue Tetrazolium Bromide (MTT) reagent kit was purchased from ABCAM, Cambridge, UK. The Annexin V-FITC Apoptosis Detection Kit and Cell cycle kits were purchased from BD Pharmingen (San Diego, CA, USA). Other chemicals used were of analytical grade.