A commercial AFM instrument (Nanoscope IIId, Bruker) equipped with either a J-scanner (125 μm × 125 μm) or a E-scanner (12 μm × 12 μm) and a liquid cell was used. All images were captured with a scan rate at 1–2 Hz. Experiments were performed in tapping mode under liquid phase. Silicon nitride cantilevers with a nominal spring constant of 0.35 N/m (SNL-10, Bruker) were used. HOPG (ZYB grade, 12 mm × 12 mm × 2 mm, Bruker) were freshly cleaved by adhesive tape before each experiment. All the real time imaging process was conducted as the following: (1) the peptide solution was slowly added into the liquid chamber through a connected tube; (2) the final concentration of the peptide in the liquid chamber was calculated by considering the previous volume of the buffer solution. AFM imaging started before adding the peptides into the liquid chamber in order to capture the whole dynamics of the peptide assembly. AFM images were processed by using NanoScope Analysis (version 1.40) that was supplied by the AFM manufacture.
Zyb grade
Manufactured by Bruker
The ZYB grade is a type of lab equipment product offered by Bruker. It is designed for specific laboratory applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach. The core function of the ZYB grade is to perform certain tasks in a laboratory setting, but further information about its intended use or capabilities is not available.
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Lab products found in correlation
2 protocols using zyb grade
1
Peptide Self-Assembly Dynamics Monitored by AFM
2
Fabrication of SSL Graphite Sheets
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The preparation process flow is shown in Supplementary Fig. 1a . First, highly oriented pyrolytic graphite (HOPG, ZYB grade, Bruker) was dissociated to obtain a clean and flat surface. Then, a layer of Ti or Cr was magnetron sputtered on the freshly cleaved surface of HOPG as an adhesion agent to increase the adhesion between graphite and subsequently deposited films. Then, a 200 nm thick cover layer is grown by magnetron sputtering. The cover layer can be made of conductive materials (such as Au, Ti, Pt, Al, etc.) or insulating materials (such as SiO2, Si3N4, etc.). The SSL materials with different overlays are shown in Fig. 2c . Next, a photoresist is spin-coated on the surface of the overlay, and an arrayed area of overlay material is obtained by direct laser writing and development. Subsequently, the overlay and adhesion were removed by ion beam etching, and the graphite layer was removed by reactive ion etching with an etching depth of 1 μm. Finally, the photoresist on the surface is removed to obtain an SSL graphite sheet array with a cover layer. The characterization of the fabricated SSL graphite with Pt film is shown in Supplementary Fig. 1b .
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