Sappβ

Low-fat (10% kcal fat; cat#D12450B) and high-fat (60% kcal fat; cat#D12492) diets were purchased from Research Diets Inc. (New Brunswick, NJ, United States). Horseradish peroxidase-conjugated donkey anti-rabbit and goat anti-mouse IgG secondary antibodies were from Jackson ImmunoResearch Laboratories (West Grove, PA, United States). Molecular weight marker, reagents, and nitrocellulose membranes for SDS-PAGE were acquired from Bio-Rad (Mississauga, ON, Canada) and GE Healthcare Life Science (cat#10600002). Western lightning Plus-ECL (PerkinElmer, 105001EA). Insulin and glucose were purchased from Eli Lilly and BioShop Reagents, respectively. All other sources are listed throughout the text. Antibodies against pro-BDNF (1:500, Santa Cruz cat #sc-65514), CREB (1:500, Cell Signaling cat #9197), pCREB (1:500, Cell Signaling cat#9196), TrkB (1:500, Cell Signaling cat#4603), pTrkB (1:500, Abcam cat#ab109684) APP (1:500 BioLegend, cat#SIG039152), pAPP Thr668 (1:500, Cell Signaling cat#6986S), BACE1 (1:500, Cell Signaling cat#5606P), sAPPα (1:500, BioLegend cat#SIG39139, and sAPPβ (1:500, BioLegend cat# SIG-39138) were obtained and used during this study.

Molecular weight marker, reagents and nitrocellulose membranes for SDS/PAGE were acquired from Bio‐Rad (Mississauga, ON, USA) and Millipore Sigma (GE10600002, Burlington, MA, USA). Antibodies against soluble amyloid precursor protein β (sAPPβ; 1 : 500; BioLegend, San Diego, CA, USA, cat# SIG‐39138), sAPPα (1 : 500, BioLegend cat#SIG39139), APP (1 : 500 BioLegend, cat#SIG039152), BACE1 (1 : 500; Cell Signaling, Whitby, Canada, cat#5606P), synaptophysin, acquired from Cell Signaling Technology (concentrations cat#5461); PSD‐95, acquired from Santa Cruz Biotechnology (concentrations cat# sc‐32290); Homer1, acquired from Santa Cruz Biotechnology (concentrations cat#sc‐136358). Horseradish peroxidase anti‐mouse and anti‐rabbit secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, West Grove, PA, USA (Donkey anti‐rabbit IgG (H + L) 711‐035‐152, Goat anti‐mouse IgG (H + L) 115‐035‐003 Jackson ImmunoResearch) and Western Lightning Plus Enhanced Chemiluminescence from Perkin Elmer (Guelph, Canada, cat#105001EA).