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2 protocols using 19 nortestosterone

1

Nandrolone Effects on Adolescent and Adult Mice

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Similar to previous experiments, adolescent and adult experimental groups received intraperitoneal (IP; Hoseini et al., 2009 (link); Witzmann, 1988 (link)) injections of nandrolone (7.5 mg/kg; 19-nortestosterone; Sigma Chemical Co. St. Louis, MO), whereas control groups received IP injections of vehicle, which consisted of sesame oil (0.02 cc/10g of body weight. As 1 mg/kg of AAS is sufficient to restore endocrine function and reproductive behaviors in rodents (Clark and Barber, 1994 (link)), the dosage used in this experiment reflects a high dose. Since periadolescence in rodents extends from postnatal (PN) day 28 to PN-60, (Spear, 2000 (link)), nandrolone injections alternated with vehicle injections, were started at PN-33 until PN-42 (Fig. 1). Furthermore, to confirm our previous experiment in adults (Parrilla-Carrero et al., 2009 (link)), adult male mice (PN 88-97) were also tested following the same CPP injection protocol as in adolescents.
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2

Analytical Standards for Veterinary and Agri-Food Applications

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Amoxicillin (99.6%), ampicillin (99.8%), benzylpenicillin (99.3%), cloxacillin (98.7%), oxacillin (98.4%), clenbuterol HCl (99.1%), isoxsuprine HCl (100%), salbutamol (99.4%), zilpaterol HCl (96.0%), ractopamine HCl (95.5%), terbutaline hemisulfate salt (100.0%), taleranol (99.5%), 19 nortestosterone (99.8%), clostebol (99.1%), boldenone (99.1%), methyltestosterone (99.5%), testosterone (100.0%), carbofuran (99.9%), carbaryl (99.9%), parathion (99.7%), malathion (99.2%), diazinon (98.3%), dimethoate (99.8%), atrazine (99.5%), cypermethrin (98.4%), permethrin (98.1%), deltamethrin (99.9%), coumaphos (99.7%), dicholphos (99.8%), chlorpyrifos (99.8%), fenvalerate (99.4%) were purchased Sigma-Aldrich (St. Louis, MO, USA). Brombuterol (98.0%), mabuterol HCl (98.0%), cimbuterol (98.0%), clenpenterol HCl (98.0%) were obtained from Witega (Berlin, Germany). Zeranol (99.9%), stanozolol (99.8%), ceftioflur (98.0%), cephalexin (96.6%), oxytetracycline (96.5%), enrofloxacin (99.74%), ciprofloxacin (98.0%), sulfadimidine (99.6%), sulfamethoxazole (99.7%), sulfadiazine (99.8%), sulfachloropiridazine (99.1%) and sulfadimethoxine (99.7%) were obtained from Dr. Ehrenstorfer GmbH (Augsburg, Germany); ochratoxin A (≥ 98.0%) and zearalenon (99.0%) were obtained from Trylogy Analytical Laboratory, Inc. (Washington, USA).
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