Antibodies were quantified using IgM and IgG quantitation sets (Bethyl Laboratories Inc) according to the manufacturer’s protocol with some adjustments: Thermo Scientific Nunc MaxiSorp 96-well ELISA plates were coated with 50 µg/mL calf thymus DNA (Thermo Fisher Scientific), phosphorylcholine (Sigma-Aldrich), hen egg lysozyme (Sigma Aldrich), glomerulus extract isolated from primary mouse glomeruli lysed in NP40 lysis buffer, or anti-C3 (clone K13/16, BioLegend for immune complex determination) in sodium carbonate coating buffer. Sera was diluted at 1:50 for quantification. Signal was quantified against IgG/IgM standard and reported as absorbance units (AU).
Anti c3 clone k13 16
Manufactured by BioLegend
Anti-C3 (clone K13/16) is a monoclonal antibody that recognizes the C3 component of the complement system. The antibody is designed for research use.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti c3 clone k13 16
1
Quantification of Antibody Isotypes
2
Quantification of Antibody Isotypes
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Antibodies were quantified using IgM and IgG quantitation sets (Bethyl Laboratories Inc. ) according to the manufacturer's protocol with some adjustments : Thermo Scientific Nunc MaxiSorp 96 well ELISA plates were coated with 50 µg/mL Calf thymus DNA (Thermo Fisher Scientific), Phosphorylcholine (Sigma Aldrich), Hen Egg Lysozyme (Sigma Aldrich), Glomerulus Extract isolated from primary mouse glomeruli lysed in NP40 lysis buffer, or anti-C3 (clone K13/16, BioLegend for immune complex determination) in sodium carbonate coating buffer. Sera was diluted at 1:50 for quantification. Signal was quantified against IgG/IgM standard and reported as absorbance units (AU).
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