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Rnase free dnase 1 step

Manufactured by Thermo Fisher Scientific
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Sourced in France

RNase-free DNase I is an enzyme used to remove DNA from RNA samples. It functions by catalyzing the hydrolytic cleavage of DNA, leaving the RNA unaffected.

Automatically generated - may contain errors

Lab products found in correlation

Trizol, by Thermo Fisher Scientific (2 mentions) Nucleospin rna clean up xs kit, by Macherey-Nagel (1 mentions) Improm 2 reverse, by Promega (1 mentions)

Close spelling variants of this product

DNAse (DNase I-RNase-Free, RNase-free DNase I RNase-free DNase DNase I (DNase Free, DNase I DNase (DNase I, RNase I DNase

2 protocols using rnase free dnase 1 step

1

Total RNA Extraction from Plant Leaves

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Leaves from three 2-week old plants plant or five spikelets from independent plants were ground in liquid nitrogen and total RNA was extracted from 0.1 g of the resulting powder using TRIzol® (Invitrogen, Life Technologies SAS, Saint-Aubin, France) followed by an RNase-free DNase I step (Ambion®, Applied Biosystems, Courtaboeuf, France) according to manufacturers’ instructions. Total RNA was further purified using the NucleoSpin RNA Clean-up XS kit (Macherey-Nagel, Hoerdt, France).
Changenet V., Macadré C., Boutet-Mercey S., Magne K., Januario M., Dalmais M., Bendahmane A., Mouille G, & Dufresne M. (2021). Overexpression of a Cytochrome P450 Monooxygenase Involved in Orobanchol Biosynthesis Increases Susceptibility to Fusarium Head Blight. Frontiers in Plant Science, 12, 662025.
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2

RNA Extraction from Plant Tissues

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Leaves, roots and spikes from 4-week-old independent plants or from infected spikes were ground in liquid nitrogen, and total RNA was extracted from 0.1 g of the resulting powder using Trizol (Invitrogen, Life Tech) followed by an RNase-free DNAse I step (Ambion, applied Biosystems) according to the manufacturers' instructions. cDNA synthesis was performed on 1 μg of total RNA using the ImProm-II reverse transcription system (Promega). The product was diluted 10 times in nuclease-free water.
Gatti M., Choulet F., Macadré C., Guérard F., Seng J.M., Langin T, & Dufresne M. (2018). Identification, Molecular Cloning, and Functional Characterization of a Wheat UDP-Glucosyltransferase Involved in Resistance to Fusarium Head Blight and to Mycotoxin Accumulation. Frontiers in Plant Science, 9, 1853.
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