Rmm csf

Manufactured by BioLegend

RmM-CSF is a recombinant mouse macrophage colony-stimulating factor produced in E. coli. It is a glycoprotein that stimulates the growth and differentiation of macrophage progenitor cells.

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Lab products found in correlation

Rmgm csf, by BioLegend (2 mentions) Astrios sorter, by Beckman Coulter (2 mentions) Streptavidin magnetic beads, by Miltenyi Biotec (1 mentions) Biotinylated mouse ly6g abs, by BioLegend (1 mentions) Macs column purification, by Miltenyi Biotec (1 mentions)

2 protocols using rmm csf

Isolation and Culture of Murine Myeloid Cells

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BM neutrophils were isolated using MACS-column purification (Miltenyi Biotec) with biotinylated mouse Ly6G Abs (BioLegend) and streptavidin magnetic beads (Miltenyi Biotec) for ex vivo study. BM monocytes, were isolated by flow cytometry-sorting as CD11b⁺Ly6G⁻Ly6C⁺ cells (Astrios sorter, Beckman Coulter). When neutrophils were compared to monocytes, flow cytometry-sorting was used to isolate neutrophils as CD11b⁺Ly6G⁺. BMDMs and BMDCs were generated by culturing BM cells with rmM-CSF (20 ng/ml, BioLegend) and rmGM-CSF (20 ng/ml, BioLegend), respectively. Complete RPMI medium was used for all cell culture studies.

Deerhake M.E., Danzaki K., Inoue M., Cardakli E.D., Nonaka T., Aggarwal N., Barclay W.E., Ji R.R, & Shinohara M.L. (2021). Dectin-1 limits autoimmune neuroinflammation and promotes myeloid cell-astrocyte crosstalk via Card9-independent expression of Oncostatin M. Immunity, 54(3), 484-498.e8.

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Isolation and Culture of Murine Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

BM neutrophils were isolated using MACS-column purification (Millentyi Biotec) with biotinylated mouse Ly6G antibodies (BioLegend) and streptavidin magnetic beads (Millentyi Biotec) for ex vivo study. For comparisons of BM neutrophils and BM monocytes, cells were isolated by FACS-sorting on CD11b + Ly6G + (neutrophils) and CD11b + Ly6G -Ly6C + (monocytes) cells (Astrios sorter, Beckman Coulter). BMDMs were generated by culturing BM cells with rmM-CSF (20 ng/ml, BioLegend) and BMDCs were generated with rmGM-CSF (20 ng/ml, BioLegend). We used complete RPMI medium for all cell culture studies.

Deerhake M.E., Danzaki K., Inoue M., Cardakli E.D., Nonaka T., Aggarwal N., Barclay W.E., Ji R., & Shinohara M.L. (2020). Dectin-1 limits central nervous system autoimmunity through a non-canonical pathway.

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