Protein lysates were prepared from control, LDLR-KO, and TAGLN-KO cells by incubation with RIPA buffer in the presence of Halt™ protease and Halt™ phosphatase inhibitors (Thermo Fisher Scientific). Prior to protein extraction for SREBP2 expression analysis, cells were incubated with 50 μg/ml of fresh human LDL for 72 h. Western blots were performed as previously described (21 (link)) using rabbit anti-human LDLR (1:500) (BioVision, Inc, Milpitas, CA), TAGLN (1:1,000), PCSK7 (1:1,000) (Cell Signaling Technology, Inc, Danvers, MA), SREBP2 (1:1,000) (Abcam, Cambridge, UK), and beta-actin (1:10,000) antibodies (GeneTex, Irvine, CA and Santa Cruz Biotechnology, Dallas, TX). The secondary antibody was goat antirabbit antibody conjugated to HRP (Abcam, Cambridge, UK).
Pcsk7
Manufactured by Cell Signaling Technology
Sourced in United Kingdom
PCSK7 is a recombinant protein enzyme that functions as a proprotein convertase. It is involved in the proteolytic processing of precursor proteins into their mature, active forms.
Automatically generated - may contain errors
Lab products found in correlation
Tagln, by Thermo Fisher Scientific (2 mentions)
Halt protease, by Thermo Fisher Scientific (2 mentions)
Rabbit anti human ldlr, by Abcam (2 mentions)
Tagln, by Cell Signaling Technology (2 mentions)
Halt phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Srebp2, by Abcam (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
2 protocols using pcsk7
1
LDLR and TAGLN Regulate SREBP2 Expression
2
Protein Expression Analysis in LDLR and TAGLN KO Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein lysates were prepared from control, LDLR-KO and TAGLN-KO cells by incubation with RIPA buffer in the presence of Halt TM protease and Halt TM phosphatase inhibitors (Thermo Fisher). Western blots were performed as previously described [19] using rabbit anti-human LDLR (1:500) (BioVision, Inc., Milpitas, CA), TAGLN (1:1000), PCSK7
(1:1000) (Cell Signaling Technology, Inc., Danvers, MA), and beta-actin (1:10,000) antibodies. The secondary antibody was donkey anti-rabbit antibody conjugated to HRP (Abcam, Cambridge, UK).
(1:1000) (Cell Signaling Technology, Inc., Danvers, MA), and beta-actin (1:10,000) antibodies. The secondary antibody was donkey anti-rabbit antibody conjugated to HRP (Abcam, Cambridge, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!