Exosome isolation kit (Qiagen, Frankfurt, Germany) was used to extract exosomes from the culture medium of MM cells according to previous studies [22 (link), 23 (link)].
Exosome isolation kit
Manufactured by Qiagen
Sourced in Germany, United States
The Exosome isolation kit is a laboratory equipment product designed to isolate and purify exosomes from various biological samples. It utilizes a proprietary technology to efficiently capture and concentrate exosomes for subsequent analysis and research applications.
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Lab products found in correlation
6 protocols using exosome isolation kit
1
Exosome Isolation from MM Cells
2
Exosome Isolation from MM Cell Culture
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Exosome isolation kit (Qiagen, Frankfurt, Germany) was used to extract exosomes from the culture medium of MM cells according to previous studies [22, 23] .
3
Exosome Isolation from MM Cell Culture
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Exosome isolation kit (Qiagen, Frankfurt, Germany) was used to extract exosomes from the culture medium of MM cells according to previous studies [22, 23] .
4
Exosome Isolation from MM Cell Culture
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Exosome isolation kit (Qiagen, Frankfurt, Germany) was used to extract exosomes from the culture medium of MM cells according to previous studies [22, 23] .
5
Exosome Isolation from Cancer Patient Plasma
6
Exosome Isolation from Cancer Patient Plasma
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The plasma samples from cancer patients and samples from healthy volunteers were separately pooled in order to perform isolation of exosomes by Exosome Isolation Kit (Exiqon, Woburn, MA, USA) or ExoQuick Plasma prep and Exosome precipitation kit (SBI, Palo Alto, CA, USA). For isolation of 1 mL exosomes from plasma using the exosome-isolation reagent, plasma was added to 17 μL of Thrombin, mixed and incubated for 5 minutes at room temperature, and then was centrifuged at 10,000 × g for 5 minutes at 4°C using Eppendorf Centrifuge 5430 R to remove the cell debris. The exosome supernatants were collected and combined with 560 μL of precipitation buffer A, vortexed for 5 seconds to mix and placed at 4°C overnight. After incubation, the mixture was centrifuged at 3200 × g for 30 minutes at 4°C to get the exosome pellet. Finally, after removing the supernatant, the exosome pellet was resuspended in 1 mL of PBS for Western blotting and frozen at −80°C.
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