Centricon ultrafilters

Manufactured by Merck Group

Centricon ultrafilters are lab equipment used for separating and concentrating macromolecules, such as proteins, from complex solutions. They work by applying pressure to force the solution through a semi-permeable membrane, which retains the macromolecules while allowing smaller molecules and solvent to pass through.

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Lab products found in correlation

Kingfisher purification system, by Thermo Fisher Scientific (2 mentions) Nuclisens kit, by bioMérieux (2 mentions)

Close spelling variants of this product

Centricon (115 citations) Ultrafilter (10 citations)

2 protocols using centricon ultrafilters

Wastewater SARS-CoV-2 Surveillance via qRT-PCR

Cited 1 time

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Wastewater specimens were collected as 24-h flow-dependent composite samples and processed as previously described (14 (link)). Debris of 100–200 mL of sewage samples was pelleted and the supernatant was concentrated by using 100 kDa Centricon ultrafilters (Millipore Sigma, https://www.emdmillipore.com); in vitro–transcribed dengue virus type-2 RNA was added as an internal extraction control. RNA was extracted by using the Nuclisens kit (bioMérieux, https://www.biomerieux.com) and KingFisher purification system (Thermo Fisher Scientific, https://www.thermofisher.com) (14 (link)). RNA was screened by quantitative reverse transcription PCR (qRT-PCR) with 5 primer–probe sets targeting the SARS-CoV-2 nucleocapsid (N) gene (N1–N3) (24 ), envelope (E) gene for all sarbecoviruses (25 (link)), and the internal control.

Izquierdo-Lara R., Elsinga G., Heijnen L., Munnink B.B., Schapendonk C.M., Nieuwenhuijse D., Kon M., Lu L., Aarestrup F.M., Lycett S., Medema G., Koopmans M.P, & de Graaf M. (2021). Monitoring SARS-CoV-2 Circulation and Diversity through Community Wastewater Sequencing, the Netherlands and Belgium. Emerging Infectious Diseases, 27(5), 1405-1415.

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SARS-CoV-2 Wastewater Surveillance Protocol

Cited 1 time

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WW samples from 9 catchment areas in Rotterdam were collected and processed as previously described (Izquierdo-Lara et al., 2021 (link); Medema et al., 2020 (link)). Briefly, 100–200 mL of sewage was collected as 24-h flow-dependent composite samples, centrifuged and the supernatant was concentrated with 100 kDa Centricon ultrafilters (Millipore Sigma); dengue virus type-2 RNA was used as an internal extraction control. RNA was extracted with the Nuclisens kit (bioMérieux) and KingFisher purification system (Thermo Fisher Scientific). RNA was screened by quantitative reverse transcription PCR (RT-qPCR) with the N2 primer/probe set targeting the SARS-CoV-2 nucleocapsid (N) gene and the internal control.

Izquierdo-Lara R.W., Heijnen L., Oude Munnink B.B., Schapendonk C.M., Elsinga G., Langeveld J., Post J., Prasad D.K., Carrizosa C., Been F., van Beek J., Schilperoort R., Vriend R., Fanoy E., de Schepper E.I., Sikkema R.S., Molenkamp R., Aarestrup F.M., Medema G., Koopmans M.P, & de Graaf M. (2023). Rise and fall of SARS-CoV-2 variants in Rotterdam: Comparison of wastewater and clinical surveillance. The Science of the Total Environment, 873, 162209.

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