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Viastain aopi reagent

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The ViaStain AOPI reagent is a laboratory product designed for use in cell viability and cytotoxicity assays. It is a fluorescent dye that can be used to stain live and dead cells. The core function of the ViaStain AOPI reagent is to provide a means of quantifying cell viability and cytotoxicity in cell-based experiments.

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Lab products found in correlation

Cellometer vision, by Revvity (2 mentions) Cellometer k2, by Revvity (1 mentions)

3 protocols using viastain aopi reagent

1

Cell Viability Determination using Cellometer

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Cells were counted on a Cellometer K2 (Nexcelom Bioscience) with the aid of Viastain AO/PI reagent (Nexcelom Bioscience #CS2-0105). Cell viability for all experiments was ≥90% prior to seeding.
Setten R.L., Chomchan P., Epps E.W., Burnett J.C, & Rossi J.J. (2021). CRED9: a differentially expressed elncRNA regulates expression of transcription factor CEBPA. RNA, 27(8), 891-906.
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2

Viable Cell Count via Cellometer

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A Cellometer Vision (Nexcelom, Lawrence, MA, USA) was used to determine total viable cell counts. Then 20 μL ViaStain AOPI reagent (Nexcelom) was added to an Eppendorf tube containing 20 μL of cells. Once mixed, 20 μL of the solution was added to a Cellometer slide, and total cells, live cells and viability were calculated.
Johnstone B.H., Miller H.M., Beck M.R., Gu D., Thirumala S., LaFontaine M., Brandacher G, & Woods E.J. (2020). Identification and Characterization of a large Source of Primary mesenchymal stem cells tightly adhered to bone Surfaces of human vertebral body marrow cavities. Cytotherapy, 22(11), 617-628.
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3

Viable Cell Count via Cellometer

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A Cellometer Vision (Nexcelom, Lawrence, MA, USA) was used to determine total viable cell counts. Then 20 μL ViaStain AOPI reagent (Nexcelom) was added to an Eppendorf tube containing 20 μL of cells. Once mixed, 20 μL of the solution was added to a Cellometer slide, and total cells, live cells and viability were calculated.
Johnstone B.H., Miller H.M., Beck M.R., Gu D., Thirumala S., LaFontaine M., Brandacher G, & Woods E.J. (2020). Identification and Characterization of a large Source of Primary mesenchymal stem cells tightly adhered to bone Surfaces of human vertebral body marrow cavities. Cytotherapy, 22(11), 617-628.
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