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Low endotoxin bovine serum albumin

Manufactured by Gemini Bio
Sourced in United States

Low-endotoxin bovine serum albumin (BSA) is a purified protein derived from bovine serum. It is a common reagent used in various laboratory applications, including cell culture, protein assays, and buffer preparations. The low-endotoxin content ensures minimal interference with biological processes.

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3 protocols using low endotoxin bovine serum albumin

1

Bronchial Epithelial Cell Culture Protocol

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Bronchial epithelial growth medium (BEGM) was obtained from Lonza (Mapleton, IL, USA). BEGM is supplemented with a number of growth factors, including bovine pituitary extract, insulin, hydrocortisone, hEGF, epinephine, triiodothyronine, transferrin, gentamicin/amphotericin-B and retinoic acid. Roswell Park Memorial Institute medium 1640 (RPMI 1640) was purchased from Invitrogen (Carlsbad, CA, USA). Low-endotoxin bovine serum albumin (BSA) and fetal bovine serum (FBS) were from Gemini Bio-Products (West Sacramento, CA, USA). Dipalmitoylphosphatidylcholine (DPPC) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Min-U-Sil was obtained from U.S. Silica (Frederick, MD, USA). All the MWCNT stock solutions were prepared using pure deionized water (DI H2O) with resistivity >18 Ω-cm.
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2

Cytotoxicity and Cell Viability Assays

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The Beta-Glo® Assay System, CytoTox 96® Non-Radioactive Cytotoxicity Assay, GSH-Glo™ Glutathione Assay, CellTiter-Glo® Luminescent Cell Viability Assay (ATP) and CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) were purchased from Promega (Madison, WI, USA); graphite flakes were provided by Asbury Graphite Mills; Hoechst 33342, FITC labeled Bovine Serum Albumin (BSA), Alexa Fluor 594-conjugated wheat germ agglutinin (WGA), Propidium iodide and Image-iT® Lipid Peroxidation Kit were purchased from Life Technologies (Grand Island, NY, USA). Min-U-Sil was obtained from U.S. Silica (Frederick, MD, USA). Bronchial epithelial growth medium (BEGM) was obtained from Lonza (Mapleton, IL, USA): this medium is supplemented with a number of growth factors, including bovine pituitary extract (BPE), insulin, hydrocortisone, hEGF, epinephrine, triiodothyronine, transferrin, gentamicin/amphotericin-B and retinoic acid. Roswell Park Memorial Institute medium 1640 (RPMI 1640) was purchased from Invitrogen (Carlsbad, CA, USA). Low-endotoxin bovine serum albumin (BSA) and fetal bovine serum (FBS) were purchased from Gemini Bio-Products (West Sacramento, CA, USA).
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3

Bronchial Epithelial Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bronchial epithelial growth medium (BEGM) was obtained from Lonza (Mapleton, IL, USA). BEGM is supplemented with a number of growth factors, including bovine pituitary extract, insulin, hydrocortisone, hEGF, epinephine, triiodothyronine, transferrin, gentamicin/amphotericin-B and retinoic acid. Roswell Park Memorial Institute medium 1640 (RPMI 1640) was purchased from Invitrogen (Carlsbad, CA, USA). Low-endotoxin bovine serum albumin (BSA) and fetal bovine serum (FBS) were from Gemini Bio-Products (West Sacramento, CA, USA). Dipalmitoylphosphatidylcholine (DPPC) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Min-U-Sil was obtained from U.S. Silica (Frederick, MD, USA). All the SWCNT, graphene or graphene oxide stock solutions were prepared using pure deionized water (DI H2O) with resistivity >18 Ω-cm.
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