Vhx 1000 digital microscope

Twigs bearing K.nepalensis (new record) were collected by Dr Juan Liu from roadside Dalbergiacochinchinensis trees at Mengzi city (22°56'N, 103°32'E), Yunnan province, China, on 15 September 2020. Fresh samples of adult females were preserved in 75% ethanol. Specimens were placed in 10% KOH for few hours and rinsed in 5–8 changes of distilled water for preparation of permanent slides as described previously (Chen et al. 2008 ). The photographs and measurements were taken with a Keyence VHX-1000 digital microscope. Terminology mainly follows Kondo and Gullan (2007) (link) and Ahmad et al. (2013b) (link). All specimens are deposited in the museum of Research Institute of Resource Insects, Kunming, China (RIRI-CAF).
More than 10 individuals were selected for observation under electron microscope. The dehydration of specimens was accomplished by passing through a series of increasing alcohol concentrations as 30%, 50%, 70%, 80%, 90%, and 95% alcohol (Mehdizadeh et al. 2014 ). They were placed on a conductive resin and gilded for 60 sec in an ion plating machine (JS-1600, Beijing Htcy Technology Co., Ltd, China) and then observed under an electron microscope (TM3000, Hitachi High-Technologies Corporation, Japan). Photographs were arranged by using Adobe Photoshop 8.0.

A Keyence VHX-1000 digital microscope (Osaka, Japan) was used to characterize the indented samples. Through the function of 3D Image Stitching, the indentation profiles were scanned, with the scanning range spanning across the lowest and the highest focusing points. The scan step size was set to be less than 2 μm.

Physcomitrella plants grown on BCDAT were cut out of plates with agar and incubated at 37°C in a 100 mM phosphate buffer with 10 mM Tris pH8.0, 1 mM EDTA pH8.0, 0.05% Triton X-100, 1 mg/mL X-GlcA (5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid) and potassium ferri/ferrocyanide using concentrations and times indicated in Figure 2 and legend. Plants were bleached in 70% ethanol and dissected and mounted in 0.3% low melting point agarose prior to imaging with a Keyence VHX-1000 digital microscope with a 0-50 X or a 50-200 X objective.

Flowering was first recorded after the ramets were randomized in the greenhouse. Some ramets had flowered with a few buds already while acclimating in the glasshouse. Flower morphology was recorded every month for twelve months. Flower buds and flowers were imaged whole and dissected longitudinally using a Keyence VHX‐1000 digital microscope. Buds and flowers, from early to late developmental stages, were dissected to determine if any developing or underdeveloped reproductive organs were present.

Overnight precultures were diluted 1:100 in LB and grown to mid-exponential phase (OD at 500 nm ~ 0.5). OD values at 500 nm values were read in a Spectronic 20D+ spectrophotometer (Thermo Fisher Scientific [Waltham, MA]) and cultures were adjusted to the same OD. Adjusted cultures were then mixed in a 2.5:97.5 ratio of fluorescent:non-fluorescent cells. Five microliters of mixture were spotted onto a bilayer plate of 45 mL (bottom layer) and 15mL (top layer) of 1% tryptone, 1% agar [Teknova (Hollister, CA) A7777] in a 10 cm × 10 cm × 1.5 cm square Petri dish (LDP [Wayne, NJ] D210–16). Plates were incubated for three days at 25°C in the dark and imaged using a VHX-1000 digital microscope (Keyence, Japan).

The scientific names of higher taxa in this catalogue follow Scholtz and Grebennikov (2016) . The structure of each entry is as follows:
– original combination of the taxon name;
– original combination and spelling of the taxon name, followed by the author, year of description, and pagination;
– type material, number of specimens (including sex, if known), and exact label data. A double slash ‘//’ indicates separate labels and single slash ‘/’ indicates lines within each label. The words in Japanese or Chinese were transcribed into Roman alphabet. Paratypes with discrepancies between collection data on the label and data quoted in the original description are indicated by ‘ [[]]’;
– remarks on types condition (given only for holotypes and neotypes);
– current taxonomic status;
– remarks, if any.
The majority of holotypes and neotypes were photographed with a Nikon D7200 camera using a Nikon AF-S DX Micro NIKKOR 40 mm f/2.8 G lens, and some types were photographed with a KEYENCE VHX-1000 Digital Microscope.

In total, 80 Celluloid sheets were aged at 70 °C and 75% RH in a fan-assisted dynamic climate chamber (MKF 115, Binder), ensuring good aeration of the samples by hanging them on glass rods. After 10 days, 40 sheets achieved a moderate condition of being slightly yellowed. After 13 days, 40 sheets showed severe discoloration and physical alteration (Figure 3). In particular, bubbles, crazes and fractures were observed in the central area of the sheets, while no visual changes were visible along the borders.
After aging, both aged and unaged sheets were kept in the dark at room temperature inside a safety storage cabinet (Q90.195.120, asecos^®) with permanent filtered (active charcoal) air ventilation.
Four analysis points were considered along the diagonal of each sheet, from its center (A) to its corner (D) (Figure 4 and Figure 5). Micro-samples with a thickness of ca. 100–200 µm for each point were taken with a surgical scalpel at the surface and at the core (at a depth of ca. 500 µm). Sampling was performed under a stereo microscope, and depth reproducibility was ensured by documenting the sheets’ section before and after sampling with a Keyence VHX-1000 digital microscope under a 5–50x magnification.