Murine colon carcinoma CT26, human bladder carcinoma J82, T24, SW1710, and UM-UC-3, breast cancer MCF-7 and T47D, colorectal cancer DLD-1, HCT15, HT29, Lovo, and HCT116, hepatoma HepG2, non-small cell lung cancer NCI-H460, ovary cancer Caov-3, prostate cancer LNCap clone FGC, and renal carcinoma Caki-1, as well as normal HUVEC, intestinal epithelial FHs74Int, and lung fibroblast MRC-5 cell lines were obtained from the National Collection of Authenticated Cell Cultures. Murine colorectal adenocarcinoma MC38 cell lines were purchased from MingzhouBio, Ningbo, Zhejiang, China. The cells were maintained in the logarithmic phase of growth in DMEM, MEM, McCoy’5a, or RPMI medium supplemented with 10% heat-inactivated FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin in an incubator at 37 °C with a humidified atmosphere of 5% CO2. Cell line identity was validated through short tandem repeat profiling, and routine mycoplasma testing was negative for contamination.
Umuc3
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The UMUC3 is a cell culture system designed for the propagation and maintenance of human bladder cancer cell lines. It provides a controlled environment for the growth and study of these cell lines, enabling researchers to conduct experiments and investigations related to bladder cancer biology and therapeutics.
Automatically generated - may contain errors
Lab products found in correlation
Sv huc 1, by National Collection of Authenticated Cell Cultures (14 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions)
Biu87, by National Collection of Authenticated Cell Cultures (7 mentions)
Um uc 3, by Thermo Fisher Scientific (6 mentions)
Sw780, by National Collection of Authenticated Cell Cultures (5 mentions)
Sv huc, by National Collection of Authenticated Cell Cultures (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (3 mentions)
Sv huc 1, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Sartorius (3 mentions)
Caov3, by National Collection of Authenticated Cell Cultures (2 mentions)
Hepg2, by National Collection of Authenticated Cell Cultures (2 mentions)
Hct116, by National Collection of Authenticated Cell Cultures (2 mentions)
Nci h460, by National Collection of Authenticated Cell Cultures (2 mentions)
Dld 1, by National Collection of Authenticated Cell Cultures (2 mentions)
Caki 1, by National Collection of Authenticated Cell Cultures (2 mentions)
Mrc 5, by National Collection of Authenticated Cell Cultures (2 mentions)
Hct15, by National Collection of Authenticated Cell Cultures (2 mentions)
38 protocols using umuc3
1
Cell Line Maintenance and Characterization
2
Bladder Cancer Cell Lines Protocol
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Human normal urothelial SV-HUC-1 cells and human bladder cancer cell lines (RT4, T24, J82, UM-UC-3, and TCCSUP) were used. The SV-HUC-1 cell line was obtained from American Type Culture Collection (ATCC), and the RT4, T24, J82, UM-UC-3, and TCCSUP cell lines were obtained from the National Collection of Authenticated Cell Cultures. SV-HUC-1 cells were cultured in F-12K medium (21127022, Gibco, New York, USA) supplemented with 10% fetal bovine serum (FBS; 1750114, Gibco). J82 and TCCSUP cells were cultured in Minimum Essential Medium (MEM) (11095080, Gibco) containing 10% FBS. T24 cells were cultured in DMEM-F12 medium (10565018, Gibco) containing 5% FBS. UM-UC-3 and RT4 cells were cultured in DMEM (11995065, Gibco) containing 10% FBS. The cells were cultured in a humid environment (5% CO2 and 95% O2) at 37°C. Plasmids were transfected in vitro using PolyJet Transfection Reagent (SignaGen Laboratories, Gaithersburg, MD, USA) according to the manufacturer's instructions, and RiboFect CP (riboBio, Guangzhou, China) was used to transfect siRNA (GenePharma, Shanghai, China). All of the siRNA sequences are shown in Table S1 .
3
Cell Line Maintenance and Characterization
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Murine colon carcinoma CT26, human bladder carcinoma J82, T24, SW1710, and UM-UC-3, breast cancer MCF-7 and T47D, colorectal cancer DLD-1, HCT15, HT29, Lovo, and HCT116, hepatoma HepG2, non-small cell lung cancer NCI-H460, ovary cancer Caov-3, prostate cancer LNCap clone FGC, and renal carcinoma Caki-1, as well as normal HUVEC, intestinal epithelial FHs74Int, and lung fibroblast MRC-5 cell lines were obtained from the National Collection of Authenticated Cell Cultures. Murine colorectal adenocarcinoma MC38 cell lines were purchased from MingzhouBio, Ningbo, Zhejiang, China. The cells were maintained in the logarithmic phase of growth in DMEM, MEM, McCoy’5a, or RPMI medium supplemented with 10% heat-inactivated FBS, 100 IU/mL penicillin, and 100 μg/mL streptomycin in an incubator at 37 °C with a humidified atmosphere of 5% CO2. Cell line identity was validated through short tandem repeat profiling, and routine mycoplasma testing was negative for contamination.
4
Bladder Cancer Cell Line Cultivation
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The human BC cell lines (5637, T24, TCCSUP, EJ, UMUC-3, BIU87 and J82) were purchased from National Collection of Authenticated Cell Cultures (Shanghai, China). The above cell lines were maintained in the Roswell Park Memorial Institute (RPMI) 1640 (Gibco; Thermo Fisher Scientific, Inc.) or minimal essential media (Gibco; Thermo Fisher Scientific, Inc.), supplemented with 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, Inc.) at 37° C with 5% CO2.
5
Bladder Cancer Cell Line Cultivation
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Bladder cancer cell lines (BIU-87, T24, J82, UM-UC-3, RT4, EJ, and TCCSUP) and one normal urothelial cell line (SV-HUC-1) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Primary normal bladder urothelial cells (NBUCs) cultures and the human uroepithelial cell line SV-HUC-1 were established as described previously [10] . The cell lines BIU-87, T24, J82, UM-UC-3, EJ, and TCCSUP were maintained in RPMI 1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco, USA), and the RT4 cell line was cultured in McCoy's 5a medium supplemented with 10% FBS. SV-HUC-1 cells were grown in F12K medium supplemented with 10% FBS.
6
Cell Culture Protocols for Bladder and Kidney
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The BCa cell lines (T24, 5637, and UM-UC-3) and the human embryonic kidney cell line HEK-293 T were obtained from the cell bank of the Chinese Academy of Sciences (Shanghai, China). The normal urothelial cell line SV-HUC-1 was obtained from the Procell Life Science and Technology (Wuhan, China). These cell lines were separately cultured in McCoy's 5a (T24), RPMI 1640 (5637 and SV-HUC-1), and DMEM (UM-UC-3 and HEK-293 T) in an incubator at 37 °C, in the presence of 5% CO2. All the culture media were supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% antibiotics (penicillin/streptomycin) (Servicebio, China).
7
Culturing Human Bladder Cancer Cell Lines
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The human BCa cell lines 5637 and UMUC3 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured at 37°C with an atmosphere of 5% CO2. The 5637 cells were cultured in 1640 (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin‐streptomycin, the UMUC3 cells were cultured in minimum Eagle's medium (MEM) (Gibco, USA) supplemented with 10% FBS and 1% penicillin‐streptomycin.
8
Bladder Cancer Tissue Sampling Protocol
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Forty tissue samples were surgically collected from participants who were treated for bladder cancer at the First Affiliated Hospital of China Medical University. All participants provided informed consent. The study was designed and all procedures were carried out in accordance with the guidelines of the Institutional Ethics Committee of China Medical University (Approval No. [2021]121). The patient characteristics regarding the samples are provided in Table S1 . HEK‐293T and bladder tumor cell lines, UMUC3 and T24, were purchased from the Cell Bank of the Chinese Academy of Sciences and cultured in high glucose DMEM medium (Gibco, Thermo Fisher Scientific, Inc) supplemented with 10% FBS (Gibco) at 37°C in 5% CO2.
9
Culturing Bladder Cancer Cell Lines
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Human bladder cancer cell lines UMUC3, HT1197, T24, J82 and human bladder epithelial cell line SV-HUC-1 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and were incubated at 37 °C in a humidified atmosphere with 5% CO2.
10
Isolation and Culture of Bladder Cell Lines
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Normal bladder urothelial cells (NBUCs) were isolated from fresh patient specimens, as described previously.31 (link) The uroepithelial cell line SV-HUC-1 and BC cell lines, including 5637, UM-UC-3, TCCSUP, T24, EJ, SCaBER, J82, and SW780, were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The T24T cell line was a gift from Dr. Guosong Jiang of Wuhan Union Hospital. All of the cell lines were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and maintained at 37°C in a 5% CO2 mammalian cell culture incubator.
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