Adventurer pro as214

The amount of wet biomass obtained after filtration was noted using a precise analytical weighing balance (Adventurer Pro AS214 d = 0.0001 g, Ohaus Europe GmbH, Greifensee, Switzerland). To determine the dry biomass weight, the wet filters were dried in a hot air oven (SNB 100, Memmert, Schwabach, Germany) at 105 °C for 17 h. The filters were weighed after keeping in desiccator for 30 min to bring it at room temperature [46 (link)].

RP-HPLC analysis was performed on a Jasco Extrema (JASCO, Inc., 28600 Mary’s court, Easton, Maryland 21601, USA) connected with an in built autosampler (AS-4150), quaternary pump (PU-4180) with an on-line degasser, column oven (CO-4061), UV/Vis detector (UV-4075) and an interface box LC-NetII/ADC. Chromatographic data was collected and analysed using ChromNAV Ver.2 software. A second HPLC system (Agilent 1100 series) equipped with variable wavelength detector (G1314A VWD), autosampler (G1313A) and isocratic pump system (G1310A). This system automated with a Chemstation (Agilent technologies) software and which was used for intermediate precision (repeatability) testing and data collection. Chromatographic separations were achieved by using SOLAS 100 Å C18 150 mm × 4.6 mm 5 µm column (Glantreo limited, Ireland). Different HPLC columns such as SOLAS ODS C18 150 mm × 4.6 mm 5 µm, SOLAS ODS C18 150 mm × 4.6 mm 3 µm, SOLAS BDS C18 150 mm × 4.6 mm 5 µm, SOLAS BDS C18 150 mm × 4.6 mm 3 µm and EIROSHELL C18 150 mm × 4.6 mm 2.6 µm (Glantreo limited) were used for this method development purpose. Each Chemical and test samples were accurately weighed using an analytical balance (Adventurer Pro AS214, Ohaus corporation, Pine Brook, NJ USA).