NCM460 and the CRC cell lines HT29, SW620, CX-1 and LOVO were obtained from the Cell Resource Center of Shanghai Institutes for Biological Sciences. HepG2, 5-8F and MDA-MB-231 were obtained from School of Basic Medical Science, Central South University. These cell lines were authenticated by short tandem repeat (STR) profiling. Before the experiment, all cells were not contaminated with mycoplasma. For the in vitro study, all of these cell lines were seeded in culture dishes or 96-well plates and allowed to grow overnight, then porric acid E with or without the CQ was added into the cell media at various concentrations and continued to be incubated for 48 h.
Ncm460
Manufactured by Cell Resource Center
Sourced in China
The NCM460 is a cell line derived from normal human colon mucosa. It is a non-transformed cell line that exhibits characteristics of normal colonic epithelial cells. The NCM460 cell line is commonly used in research related to colon and gastrointestinal physiology and pathology.
Automatically generated - may contain errors
2 protocols using ncm460
1
Cell line characterization and treatment
2
Culturing Normal and CRC Cell Lines
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The normal human colon mucosal epithelial cell line NCM460 and the CRC cell lines LS174T, DLD1, HT29, HCT116, SW480, and SW620 were purchased from the Shanghai Institute of Life Sciences Cell Resource Center in Shanghai, China. All cell lines were cultured in DMEM (HyClone) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Gibco). All cell cultures were maintained at 37 °C in a humidified atmosphere with 5% CO2.
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