The levels of ITGαV (Santa Cruz. Cat. Sc-10729; dilution 1:200), fibronectin (Santa Cruz. Cat. Sc-80205; dilution 1:1000), vimentin (Santa Cruz. Cat. Sc-373717; dilution 1:1000) and MMP-9 (Santa Cruz Cat. SC-21733; dilution 1:200) were measured in A549 lysates by immunoblotting using denaturing polyacrylamide gels at 12%. The same proteins were measured in MLE-12 and RLE-6TN lysates: vimentin (Abcam, Cat. Ab92547; dilution 1:200), fibronectin (Abcam, ab45688; dilution 1:200), ITGαV (Santa Cruz, Cat. sc-10729; dilution 1:200), and MMP-9 (Santa Cruz, Cat. sc-6840; dilution 1:200). Gels were transferred to nitrocellulose membrane (Osmonics, Westboroug, MA) and blocked with fat free milk at 5% in TBS/0.1% Tween. Membranes were incubated with primary antibody specific for each protein overnight. The secondary antibody was used coupled to peroxidase (anti-rabbit, anti-goat or anti-mouse, Zymed, CA). Proteins were visualized by chemiluminescence (Pierce, USA) and normalized against beta-tubulin. (Biolegend Cat. 622102).
Itgαv
Manufactured by Santa Cruz Biotechnology
The ITGαV is a laboratory instrument designed for use in biotechnology research. It is a tool that enables the measurement and analysis of specific cellular and molecular components. The core function of the ITGαV is to facilitate the quantification and study of targeted analytes, supporting researchers in their scientific investigations. However, a more detailed description cannot be provided while maintaining an unbiased and purely factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Vimentin, by Abcam (1 mentions)
Fibronectin, by Abcam (1 mentions)
Fibronectin, by Santa Cruz Biotechnology (1 mentions)
Vimentin, by Santa Cruz Biotechnology (1 mentions)
Mmp 9, by Santa Cruz Biotechnology (1 mentions)
Phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions)
Ecl system, by Cytiva (1 mentions)
Itg β5, by Cell Signaling Technology (1 mentions)
Anti phospho fak, by Cell Signaling Technology (1 mentions)
Total fak, by Merck Group (1 mentions)
Hsp90, by Santa Cruz Biotechnology (1 mentions)
2 protocols using itgαv
1
Quantitative Immunoblotting of Lung Cell Markers
2
Western Blot Analysis of Protein Expression
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Western blots were carried out as previously described 46 . Cell lysates (30µg) were separated by SDS-PAGE, transferred onto a PVDF membrane and then probed with antibodies to MITF (Abcam, Ab12039), ASAH1 (Sigma, HPA005468), ERK2 (Santa Cruz biotechnology, clone D-2), phospho-ERK1/2 (Thr202/Tyr204) (Cell Signaling Technology Inc., #2370), anti-phosphoFAK (Cell Signaling Technology Inc., #3283), total FAK (Millipore, clone 4.47), p27 (Ozyme, #3686), ITGβ5 (Cell Signaling Technology Inc., D24A5), ITGαV (Santa Cruz biotechnology, p2W7), to integrin alpha V+beta 5 (Abcam, ab24694), E-cadherin (Ozyme, #5296) and HSP90 (Santa Cruz biotechnology, sc-13119). Horseradish peroxidase-conjugated anti-rabbit or antimouse antibodies were from Dakopatts (Glostrup, Denmark). Proteins were visualized with the ECL system (Amersham). The western blots shown are representative of at least 3 independent experiments.
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