Anti beta actin

Manufactured by LI COR

Anti-beta actin is a monoclonal antibody that binds specifically to the beta-actin protein, a ubiquitous cytoskeletal protein found in eukaryotic cells. It is commonly used in Western blotting and immunocytochemistry applications to detect and quantify the presence of beta-actin in biological samples.

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Lab products found in correlation

Anti mouse f4 80, by BioLegend (1 mentions) Hydroxyproline assay kit, by Cell Biolabs (1 mentions) Anti human fibrinogen, by Abcam (1 mentions) Odyssey fc, by LI COR (1 mentions) Mini protean tgx stain free protein gel, by Bio-Rad (1 mentions) Revert total protein stain, by LI COR (1 mentions) Anti gapdh, by LI COR (1 mentions) Intercept blocking buffer, by LI COR (1 mentions) Empiria studio software, by LI COR (1 mentions) Anti synaptophysin, by Abcam (1 mentions)

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Beta-actin (2 citations)

2 protocols using anti beta actin

Antibody Profiling for Tissue Analysis

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Primary antibodies used for western blot analysis were: anti-beta actin (LI-COR), anti-human fibrin(ogen) (Abcam), anti-casein (ABBIOTEC), anti-Mcl-1 and anti-cleaved caspase 3 (Cell Signaling Technology) and anti-entactin/nidogen (Millipore).
Primary antibodies used for immunohistochemical staining of paraffin embedded tissue were: anti-collagen IV and anti-α-smooth muscle actin (Abcam), anti-laminin 1 (R&D Systems), anti-mouse β₁ integrin (Chemicon), anti-mouse F4/80 (Biolegend),, anti-human fibrinogen (Dako), and anti-mouse Plg-R_KT, produced in our laboratory [15 (link)]. For immunohistochemistry of frozen sections, anti-Ser₂₈-phosphorylated histone H3 was from Sigma.
Mouse prolactin and uPA ELISA kits and an ELISA kit recognizing both fibrinogen and fibrin species were from Innovative Research, Inc. The mouse D-dimer ELISA kit was from LifeSpan Bioscience. The hydroxyproline assay kit was from Cell Biolabs.

Miles L.A., Baik N., Bai H., Makarenkova H.P., Kiosses W.B., Krajewski S., Castellino F.J., Valenzuela A., Varki N.M., Mueller B.M, & Parmer R.J. (2018). The Plasminogen Receptor, Plg-RKT, is Essential for Mammary Lobuloalveolar Development and Lactation. Journal of thrombosis and haemostasis : JTH, 16(5), 919-932.

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Quantitative Analysis of Synaptic Proteins

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Equal volumes of protein extracts were loaded in each well of a 4–20% Mini-PROTEAN TGX Stain-Free Protein Gel (BioRad), separated by electrophoresis, and transferred onto an FL-PVDF membrane (Millipore). Nonspecific binding sites were blocked in Intercept Blocking Buffer (LI-COR) for 1 h. Blots were probed overnight at 4°C. Bands were visualized by two-channel multiplex fluorescence (LI-COR Odyssey Fc). Quantification of western blots was performed using LI-COR Empiria Studio software. Samples were normalized using Revert Total Protein Stain (LI-COR). Antibodies used in this study were anti-PSD-95 (Antibodies Inc.), anti-synaptophysin (Abcam), anti-GAPDH (LI-COR), and anti-beta-actin (LI-COR) (Supplementary Table 3). Individuals performing the immunoblotting experiments were blinded to the research participants’ demographic, clinical, and neuropathologic characteristics.

Lilek J., Ajroud K., Feldman A.Z., Krishnamachari S., Ghourchian S., Gefen T., Spencer C.L., Kawles A., Mao Q., Tranovich J.F., Jack CR J.r., Mesulam M.M., Reichard R.R., Zhang H., Murray M.E., Knopman D., Dickson D.W., Petersen R.C., Smith B., Ashe K.H., Mielke M.M., Nelson K.M, & Flanagan M.E. (2023). Accumulation of pTau231 at the Postsynaptic Density in Early Alzheimer’s Disease. Journal of Alzheimer's Disease, 92(1), 241-260.

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