Streptavidin magnetic nanoparticles (SMNPs) (1.0 μm in diameter, 10 mg/mL) were obtained from Invitrogen Biotechnology Co., Ltd. (Carlsbad, CA, USA). Salmon sperm DNA, tRNA, and Tris were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). AuNPs (15 nm) were acquired from Jieyi Biotechnology Co., Ltd. (Shanghai, China). Ethylene diamine tetraacetic acid (EDTA) was received from Saiguo Biotech Co., Ltd. (Guangzhou, China). The sample pad, conjugate pad, nitrocellulose membrane, absorption pad, and polyvinyl chloride pad were provided from Hangzhou Goodhere Biotechnology Co., Ltd. (Hangzhou, China). Aptamers and ssDNA were synthesized by Sangon Biotech Co., Ltd., (Shanghai, China); sequences are shown in Table 1 . Polyvinylpyrrolidone (PVP) and sodium dodecyl sulfate (SDS) were also obtained from Sangon Biotech Co., Ltd. Tween-20, D-trehalose, Tris-HCl, and sucrose were acquired from Yihui Biological Technology Co., Ltd. (Shanghai, China). Trisodium citrate was purchased from Sinopharm Chemical Reagent Company (Beijing, China). Streptavidin was acquired from Sigma Co., Ltd (Silicon Valley, CA, USA). Disodium phosphate, sodium dihydrogenphosphate was obtained by Tianli Chemical Reagent Co., Ltd. (Tianjin, China). All chemicals used in this study were of analytical reagent grade.
Salmon sperm dna
Manufactured by Solarbio
Sourced in China
Salmon sperm DNA is a type of DNA extracted from the sperm cells of salmon. It is commonly used as a model system for DNA-related research and applications in molecular biology and biotechnology. Salmon sperm DNA serves as a source of genetic material for various experimental purposes, such as DNA hybridization, transfection, and DNA precipitation.
Automatically generated - may contain errors
Lab products found in correlation
Sodium dodecyl sulfate (sds), by Sangon (1 mentions)
Trisodium citrate, by Sinopharm (1 mentions)
Polyvinylpyrrolidone pvp, by Sangon (1 mentions)
Cutsmart buffer, by New England Biolabs (1 mentions)
Milli q, by Merck Group (1 mentions)
Streptavidin magnetic beads, by New England Biolabs (1 mentions)
Nb bbvci nicking endonuclease, by New England Biolabs (1 mentions)
Millipore q water, by Merck Group (1 mentions)
3 protocols using salmon sperm dna
1
Streptavidin Magnetic Nanoparticle-based Assay
2
Magnetic Bead-based DNA Nicking Assay
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Streptavidin-magnetic beads (1 μm diameter, 4 mg mL−1, an aqueous suspension containing 0.1% bovine serum albumin, pH 7.4, 0.05% Tween-20, and 0.05% NaN3), nicking endonuclease (Nb.BbvCI) and 10× CutSmart™ buffer (20 mM, Tris-acetate, 500 mM potassium acetate, 10 mM magnesium acetate, and 100 μg mL−1 BSA, pH 7.9) were purchased from New England Biolabs (Beijing, China). Gold View I (GV I) (10 000×) used in this study was provided by Solarbio Co. Ltd. (Xiamen, China). The DL 200 DNA marker was ordered from Takara (Dalian, China). Salmon sperm DNA was obtained from Solarbio Inc. (Beijing, China). All oligonucleotides were synthesized and purified via HPLC by Sangon Biotechnology Co. Ltd. (Shanghai, China), and the corresponding sequences are illustrated in Table S1 (ESI).† All oligonucleotides were dissolved in tris-ethylenediaminetetraacetic acid (TE) buffer (pH 8.0, 10 mM Tris–HCl, 1 mM EDTA) and stored at −20 °C for further use.
Moreover, 0.1 M phosphate-buffered solution (PBS) included 136.89 mM NaCl, 2.67 mM KCl, 8.10 mM Na2HPO4 and 1.76 mM KH2PO4 (pH 7.4). The reaction buffer contained 10 mM Tris–HCl, 1 mM EDTA and 12.5 mM MgCl2 (pH 8.0). Other chemicals (analytical grade) were obtained from standard reagent suppliers. Millipore-Q water (≥18 MΩ, Milli-Q, Millipore) was used throughout the experiments.
Moreover, 0.1 M phosphate-buffered solution (PBS) included 136.89 mM NaCl, 2.67 mM KCl, 8.10 mM Na2HPO4 and 1.76 mM KH2PO4 (pH 7.4). The reaction buffer contained 10 mM Tris–HCl, 1 mM EDTA and 12.5 mM MgCl2 (pH 8.0). Other chemicals (analytical grade) were obtained from standard reagent suppliers. Millipore-Q water (≥18 MΩ, Milli-Q, Millipore) was used throughout the experiments.
3
Evaluating DNA-binding Ability of AMSIN
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The inhibition‐zone assay was used to examine the DNA‐binding ability of AMSIN. The rationality behind this experiment is that if a reduction in the inhibition‐zone size in the presence of DNA, it would indicate that the peptide binding to the DNA causes the loss of antibacterial activity. To this end, 1.0 nmol AMSIN mixed with the salmon sperm DNA (Solarbio Life Sciences, Beijing, China) (a final concentration of 6.6 mg/ml) was added to a well of the MRSA P1374 plate and incubated at 37°C overnight. Inhibition zones were recorded and compared with the control without the DNA.
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