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Nanofil sub microliter injection syringe

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The NanoFil Sub-Microliter Injection syringe is a precision instrument designed for accurate and controlled delivery of small liquid volumes. It is a handheld syringe with a glass barrel and a metal needle, capable of delivering volumes ranging from 100 nanoliters to 10 microliters.

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Lab products found in correlation

Injection pump, by Harvard Apparatus (4 mentions) Aav9 hsyn gcamp6f, by Addgene (1 mentions)

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Nanofil syringe (110 citations) NanoFil (30 citations) Sub-microliter injection syringe (2 citations)

4 protocols using nanofil sub microliter injection syringe

1

Intracerebral Virus Injection Surgery

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Puralube vet ointment was applied to the eyes and 0.2mg/kg meloxicam was administered intraperitoneally using a 1mL syringe. Hair from the scalp was trimmed, and the area was sterilized using povidone-iodine swabs and subsequently ethanol swabs. An incision covering the anteroposterior extent was made to allow access to the skull. Injection sites were accessed using a dental drill which made 0.5mm holes through the skull. All virus was injected using a 35G beveled needle in a 10ul NanoFil Sub-Microliter Injection syringe (World Precision Instruments) controlled by an injection pump (Harvard Apparatus) at a rate of 100nl/min. After all viral delivery, an additional 5–10 mins delay was applied to avoid backflush before slowly removing the injection needle. Animals that required cannulas or GRIN lenses were implanted immediately following viral injection. Following surgery, mice were allowed to recover in a single housed cage for up to 12 hours, and were given meloxicam tablets. Mice were typically housed for three weeks to allow for adequate expression before behavioral testing or histology.
Toader A.C., Regalado J.M., Li Y.R., Terceros A., Yadav N., Kumar S., Satow S., Hollunder F., Bonito-Oliva A, & Rajasethupathy P. (2023). Anteromedial Thalamus Gates the Selection & Stabilization of Long-Term Memories. bioRxiv.
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2

Viral Injection and Surgical Implantation

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Puralube vet ointment was applied to the eyes and 0.2mg/kg meloxicam was administered intraperitoneally using a 1mL syringe. Hair from the scalp was trimmed, and the area was sterilized using povidone-iodine swabs and subsequently ethanol swabs. An incision covering the anteroposterior extent was made to allow access to the skull. Injection sites were accessed using a dental drill which made 0.5mm holes through the skull. All virus was injected using a 35G beveled needle in a 10ul NanoFil Sub-Microliter Injection syringe (World Precision Instruments) controlled by an injection pump (Harvard Apparatus) at a rate of 100nl/min. After all viral delivery, an additional 5–10 mins delay was applied to avoid backflush before slowly removing the injection needle. Animals that required cannulas or GRIN lenses were implanted immediately following viral injection. Following surgery, mice were allowed to recover in a single housed cage for up to 12 hours, and were given meloxicam tablets. Mice were typically housed for three weeks to allow for adequate expression before behavioral testing or histology.
Toader A.C., Regalado J.M., Li Y.R., Terceros A., Yadav N., Kumar S., Satow S., Hollunder F., Bonito-Oliva A, & Rajasethupathy P. (2023). Anteromedial Thalamus Gates the Selection and Stabilization of Long-Term Memories. Cell, 186(7), 1369-1381.e17.
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3

Viral Injection and Surgical Implantation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Puralube vet ointment was applied to the eyes and 0.2mg/kg meloxicam was administered intraperitoneally using a 1mL syringe. Hair from the scalp was trimmed, and the area was sterilized using povidone-iodine swabs and subsequently ethanol swabs. An incision covering the anteroposterior extent was made to allow access to the skull. Injection sites were accessed using a dental drill which made 0.5mm holes through the skull. All virus was injected using a 35G beveled needle in a 10ul NanoFil Sub-Microliter Injection syringe (World Precision Instruments) controlled by an injection pump (Harvard Apparatus) at a rate of 100nl/min. After all viral delivery, an additional 5–10 mins delay was applied to avoid backflush before slowly removing the injection needle. Animals that required cannulas or GRIN lenses were implanted immediately following viral injection. Following surgery, mice were allowed to recover in a single housed cage for up to 12 hours, and were given meloxicam tablets. Mice were typically housed for three weeks to allow for adequate expression before behavioral testing or histology.
Regalado J.M., Asensio A.C., Haunold T., Toader A.C., Li Y.R., Neal L.A, & Rajasethupathy P. (2024). Neural activity ramps in frontal cortex signal extended motivation during learning. bioRxiv.
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4

Stereotactic Viral Injections for Optogenetic and Chemogenetic Manipulation

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All surgical procedures and viral injections were carried out under protocols approved by the Rockefeller University IACUC. Mice were anesthetized with 1–2% isoflurane and placed in the stereotactic apparatus (Kopf) under a heating pad. Paralube vet ointment was applied on the eyes to prevent drying. Virus was injected using a 34–35 G beveled needle in a 10ul NanoFil Sub-Microliter Injection syringe (World Precision Instruments) controlled by an injection pump (Harvard Apparatus). All viruses were injected at the rate 75nl/min (unless mentioned otherwise), and the needle was removed 10mins after the injection was done to prevent backflow of the virus.

For imaging, 500–700nl of AAV1(or AAV9)-hSyn-GCaMP6f (Addgene, Cat No. 100837-AAV1 (or AAV9); titer: ~1.5*1013 vg/mL) was injected in AC or CA1.

For inhibition-imaging experiments, ~300–400ul of a soma targeted AAV1-stGtACR2 under a CamKII promoter (Addgene, Cat No. 105669-AAV1; titer: ~8*1012 vg/mL) was injected unilaterally in AC/LEC.

For optogenetic experiments, stGtACR2 was injected bilaterally in AC/LEC with the same amount and titer, with CamKII-mCherry in control cohorts (Addgene Cat No. 114469-AAV1; titer: ~9*1012 vg/mL).

For chemogenetic inhibition, Gi coupled DREADD AAV9-hSyn-hM4D(Gi)-mCherry (Addgene, Cat No. 50475-AAV9; titer: : ~1*1013 vg/mL) virus was injected in CA1 (A/P: -1.5mm, M/L: ±1.5mm, D/V: -1.6mm) bilaterally.

Yadav N., Noble C., Niemeyer J.E., Terceros A., Victor J., Liston C, & Rajasethupathy P. (2022). Prefrontal Feature Representations Drive Memory Recall. Nature, 608(7921), 153-160.
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