1 ethyl 3 3 dimethylaminopropyl carbodiimide edc

Manufactured by Sinopharm

Sourced in China, United States

1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) is a common coupling agent used in chemical synthesis. Its core function is to facilitate the formation of amide bonds between carboxylic acids and primary amines.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) N hydroxysuccinimide, by Sinopharm (2 mentions) Dimethyl octadecyl epoxypropyl ammonium chloride, by Sinopharm (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Cholesterol, by Sinopharm (1 mentions) Prussian blue staining kit, by Solarbio (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Tianamp genomic dna kit, by Tiangen Biotech (1 mentions) Dichloromethane, by Sinopharm (1 mentions) N hydroxysuccinimide nhs, by Sinopharm (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Hoechst 33342, by Beyotime (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Beyotime (1 mentions) Polycaprolactone, by Merck Group (1 mentions) Lb broth agar, by Sangon (1 mentions) 2 n morpholino ethane sulfonic acid mes, by Maclin Biochemical Technology (1 mentions) Lb broth, by Sangon (1 mentions) Chitosan, by Maclin Biochemical Technology (1 mentions) Gelatin type a, by Merck Group (1 mentions) N hydroxysuccinimide nhs, by Adamas (1 mentions)

4 protocols using 1 ethyl 3 3 dimethylaminopropyl carbodiimide edc

Multimodal Labeling for Cell Characterization

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Fe₃O₄ solution, carboxymethyl chitosan hexadecyl quaternary ammonium salt (HQCMC), CK8/18/19 (CK)-FITC (Fluorescein Isothiocyanate), CD45-PE, DAPI, Ep-LMS, and Vi-LMS were purchased from Huzhou Lieyuan Medical Laboratory Co., Ltd. A Prussian blue staining kit was purchased from Solarbio. DMEM, fetal bovine serum and trypsin were purchased from Gibco, and GPC3 antibody was purchased from Abcam. 1,2-Dioleoyl phosphatidylcholine (DOPC), dimethyl octadecyl epoxy propyl ammonium chloride (GHDC), cholesterol, dichloromethane, N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and other commonly used reagents were all purchased from Sinopharm Group. The TIANamp Genomic DNA Kit was purchased from Tiangen Biotech (Beijing).

Huang X.Y., Li F., Li T.T., Zhang J.T., Shi X.J., Huang X.Y., Zhou J., Tang Z.Y, & Huang Z.L. (2023). A clinically feasible circulating tumor cell sorting system for monitoring the progression of advanced hepatocellular carcinoma. Journal of Nanobiotechnology, 21, 25.

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Synthesis and Characterization of HA-PEG-DOX Conjugates

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The HA-PHis was synthesized according to the previously reported procedures. D-α-tocopheryl acid succinate was obtained from Jiangsu Xixin International Co., Ltd. (Suqian, People’s Republic of China). Methoxyl PEG2000-amine (mPEG2k-NH₂) and amine-PEG2000-carboxymethyl (NH₂-PEG2k-COOH) were bought from Yare Biotech (Shanghai, People’s Republic of China). Her2 peptide (YCDGFYA-CYMDV, Figure 1A) was synthesized by China Peptides Co., Ltd. (Shanghai, People’s Republic of China). DOX hydrochloride was purchased from Huafeng United Technology (Beijing, People’s Republic of China). N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, People’s Republic of China), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and Hoechst 33342 were bought from Beyotime (Shanghai, People’s Republic of China). Dulbecco’s Modified Eagle’s Medium (DMEM), Roswell Park Memorial Institute (RPMI)-1640 medium, fetal bovine serum (FBS), and penicillin–streptomycin solution were purchased from Gibco^® BRL (Thermo Fisher Scientific, Waltham, MA, USA). All the other chemicals and buffer solution components were analytical-grade preparations.

Chen Q., Long M., Qiu L., Zhu M., Li Z., Qiao M., Hu H., Zhao X, & Chen D. (2016). Decoration of pH-sensitive copolymer micelles with tumor-specific peptide for enhanced cellular uptake of doxorubicin. International Journal of Nanomedicine, 11, 5415-5427.

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Biomimetic Wound Dressing Fabrication

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Polycaprolactone (PCL, Mn = 80 kDa) and type A gelatin (300 g Bloom) were obtained from Sigma Aldrich (St. Louis, Missouri, USA). Chitosan (CS, 90% deacetylated powder, Mw = 200 kDa), 4-octenyl itaconate (OI), and 2-(N-morpholino) ethanesulfonic acid (MES) were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Glacial acetic acid and 2,2,2-trifluoroethanol (TFE) were obtained from Meryer Chemical Technology Co., Ltd. (Shanghai, China). Adhesive bandage was obtained from Hainuo Medical Technologies Co., Ltd. (Qingdao, China). 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). N-hydroxysuccinimide (NHS) was obtained from Adamas-beta (Shanghai, China). LB Broth and LB Broth Agar were purchased from Sangon Biotech (Shanghai, China).

He J., Zhou S., Wang J., Sun B., Ni D., Wu J, & Peng X. (2024). Anti-inflammatory and anti-oxidative electrospun nanofiber membrane promotes diabetic wound healing via macrophage modulation. Journal of Nanobiotechnology, 22, 116.

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Magnetic Nanoparticle-Based Cell Isolation

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DMEM culture medium, fetal bovine serum and trypsin (Gibco); Fe₃O₄ solution, hexadecyl-quaternized (carboxymethyl) chitosans (HQCMC), CK-FITC, CD45-PE and DAPI (Huzhou Lieyuan Medical Laboratory Co., Ltd.); 1,2 dioleolyl-sn-glycero-3-phosphatidylcholine (DOPC), glycidyl hexadecyl dimethylammonium chloride (GHDC), cholesterol, N-hydroxysuccinimide (NHS), and 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide (EDC) (Sinopharm); EpCAM antibody and Vimentin antibody (CST); BI-90Plus Nanoparticle Size Analyzer/Zeta potentiometer (Brookhaven, USA); OLYMPUS B×61 fluorescence microscope (Olympus, Japan); and LDJ9600-1 magnetic property measurement system (MPMS) (Digital Instruments Inc., USA).

Huang C., Ding S., Huang C., Pan F., Liu X., Zhang H., Zhou J., Liang X., Wang X, & Song P. (2021). Distribution and Clinical Analysis of EpCAM+/Vimentin+ Circulating Tumor Cells in High-Risk Population and Cancer Patients. Frontiers in Oncology, 11, 642971.

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