Diphenyltetrazolium bromide mtt

The primary antibodies used in this study were at the dilutions of 1:1000 as follows: CD229 (ab103172, Abcam, UK); RASAL3 (ARP79758_P050, Aviva Systems Biology, USA); Flag (14793S, Cell Signaling Technology, USA); ERK (4695S, Cell Signaling Technology, USA); p-ERK (4370S, Cell Signaling Technology, USA); GAPDH (5174S, Cell Signaling Technology, USA). The second antibodies Goat anti-Rabbit IgG (H+L) HRP (FMS-Rb01, Fcmacs) and Goat anti-Mouse IgG (H+L) HRP (S0002, Affinity) were in 5000 diluted concentrations. Puromycin was obtained from Merck KGaA (Darmstadt, Germany). Diphenyltetrazolium Bromide (MTT) was purchased from Solarbio (Shanghai, China). PKH26 was purchased from Sigma-Aldrich (Lot#SLBP9768V, SIGMA, USA).

The primary antibodies used in this study were at the dilutions of 1:1000 as follows: SFRS8 (24705‐1‐AP, ProteinTech Group, China; ab72044, Abcam, UK); poly ADP‐ribose polymerase (PARP) (9542S, Cell Signaling Technology, USA); β‐catenin (51067‐1‐AP, ProteinTech Group, China); β‐actin (4970S, Cell Signaling Technology, USA); Alix (2171S, Cell Signaling Technology, USA); CD9 (13174S, Cell Signaling Technology, USA); Calnexin (10427‐2‐AP, ProteinTech Group, China); Ubiquitin (10201‐2‐AP, ProteinTech Group, China); HA (51064‐2‐AP, ProteinTech Group, China); DYKDDDDK (98533S, Cell Signaling Technology, USA). The anti‐DYKDDDDK antibody (101274‐mm05t, Sino Biological, China) was at the 1:50 dilution, and Goat pab to Ms IgG (FITC) (ab6785, Abcam, UK) was at the 1:200 dilution. The second antibodies Goat anti‐Rabbit IgG(H+L) HRP (FMS‐Rb01, Fcmacs) and goat anti‐Mouse IgG (H+L) HRP (S0002, Affinity) were in 5 000 diluted concentrations.
Doxycycline (DOX), rabbit IgG (a7016) and mouse IgG (a7028) were purchased from the Beyotime (Shanghai, China). Puromycin was obtained from Merck KGaA (Darmstadt, Germany). Diphenyltetrazolium Bromide (MTT) was purchased from Solarbio (Shanghai, China). Methyl 3‐{[(4‐methylphenyl) sulfonyl] amino}benzoate (MSAB) was purchased from Selleck (Shanghai, China). GW4869 was purchased from MedChemExpress (Monmouth Junction, NJ, USA).