Mice were placed, single-housed, in metabolic cages (Sable Systems International, Promethion high-definition behavioral phenotyping system) housed in thermal cabinets set to 30°C with a 12-hour light and 12-hour dark schedule. The following morning at Zeitgeber (ZT) ZT3, mice were injected with vehicle (saline) and placed back in the metabolic cages to monitor the stress response to i.p. injection. The next morning (at ZT3), the same volume of the β3-adrenoreceptor agonist, CL 316,243, was administered i.p. at 1mg/kg, and mice were placed back in the metabolic cages. Saline and Cl 316,243 responses were followed every 3 minutes for 90 minutes. Mass-dependent variables (VO2, VCO2, and energy expenditure) were not normalized to body weight. Oxygen consumption (VO2), carbon dioxide expiration (VCO2), energy expenditure (kcal), respiratory exchange ratio, and total physical movement (meters; measured by infrared beam breaks) were recorded every 3 min using Sable Systems data acquisition software (IM-3 v.20.0.3). Data were parsed/analyzed using Sable Systems International MacroInterpreter software (v.2.41) using One-Click Macro (v2.37).
Promethion high definition behavioral phenotyping system
Manufactured by Sable Systems
The Promethion high-definition behavioral phenotyping system is a laboratory equipment used for comprehensive animal behavior analysis. It provides detailed measurements and observations of various animal behaviors in a controlled environment.
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Lab products found in correlation
2 protocols using promethion high definition behavioral phenotyping system
1
β3-Adrenoreceptor Agonist Effects on Metabolic Physiology
2
Metabolic Profiling of Mice on Diets
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For simultaneous measurements of whole body metabolic rates, mice fed a ND or a HFD were housed individually in metabolic cages (Sable Systems International, Promethion high-definition behavioral phenotyping system) maintained on a 12 h:12 h light:dark cycle at 22 °C with free access to water and food. VO2, VCO2, RER, locomotor and ambulatory activity, as well as food/water intake were measured after at least 24 h acclimation. For C29 injections, mice were subjected to daily intraperitoneal injection of vehicle or C29 at ZT 10 and placed back in the metabolic cages. CalR59 (link) (version 1.3) was used to generate hourly plots with gray squares denoting night periods.
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