Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The antibody against survivin was purchased from Cell Signaling Technology (Danvers, MA, USA). PI3 Kinase inhibitor Ly294002 was purchased from Calbiochem (San Diego, CA). The antibody against cleaved PARP (24 kDa) was purchased from Epitomics (Burlingame, CA, USA). Antibodies against fibronectin, snail, dysadherin, STAT3, phosphorylated STAT3 (Y705), vimentin, and MCP-1 were purchased from Abcam (Cambridge, UK). The antibody against E-cadherin was purchased from BD Biosciences (San Jose, CA, USA).
Antibody against survivin
Manufactured by Cell Signaling Technology
Sourced in United States
Antibody against survivin is a laboratory reagent used to detect the expression of the survivin protein in biological samples. Survivin is a member of the inhibitor of apoptosis (IAP) protein family and plays a role in regulating cell division and apoptosis. This antibody can be used in various analytical techniques, such as western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of survivin in different cell types and tissues.
Automatically generated - may contain errors
2 protocols using antibody against survivin
1
Survivin Pathway Modulation Assay
2
Survivin Expression in GIST Tissues
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Tissue microarray sections (4‐μm thickness) were obtained from paraffin‐embedded GIST tissues from 113 patients. Serial sections were manually stained with antibody against survivin (1:1,200, Cell Signaling Technology). Survivin expression was evaluated based on H‐scores, which were calculated using both expression intensity and proportion of expression in tumors according to the following equation: (1 × [% cells 1+] + 2 × [% cells 2+] + 3 × [% cells 3+]), where 1, 2 and 3 represent the relative intensity of survivin expression. H‐scores were used to group patients according to strong, moderate or weak survivin expression. Fisher's exact test, the χ2‐test, Kaplan–Meier survival analysis, Pearson's correlation analysis and Cox proportional hazards analysis were used to analyze the relationships between survivin expression and clinicopathological parameters (SPSS software, SPSS, Inc., Chicago, IL, USA). DAVID software (Database for Annotation, Visualization an Integrated Discovery, v6.7; http://david.abcc.ncifcrf.gov ) and BINGO 2.3 plugin for Cytoscape software (http://www.psb.ugent.be/cbd/papers/BiNGO/Home.html ) were used for pathway and gene ontology analysis. Putative survivin binding regions of KIT gene were selected using MethPrimer (http://www.urogene.org/cgi-bin / methprimer/methprimer.cgi).
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