Biochemical studies of E10.5 Nfatc1Cre;Krit1fl/fl hearts were performed as previously described (Kleaveland et al., 2009 (link); Zheng et al., 2010 (link)). The following antibodies were used for immunonlotting: rabbit anti-Gapdh (1:5000, Cell Signaling), rabbit anti-pERK5 (1:1000, Cell Signaling), rabbit anti-Adamts5 (1:1000, Abcam), rabbit anti-DPEAAE (1:1000, Pierce-Antibodies). Identification of BirA-MEKK3 interacting proteins is described in Supplemental Materials and Methods .
Rabbit anti perk5
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-pERK5 is a primary antibody that recognizes the phosphorylated form of the extracellular signal-regulated kinase 5 (ERK5) protein. ERK5 is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in cellular processes such as proliferation, differentiation, and survival. The antibody can be used to detect the phosphorylated state of ERK5 in various cellular and experimental contexts.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti erk5, by Cell Signaling Technology (2 mentions)
Rabbit anti adamts 5, by Abcam (1 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (1 mentions)
Hrp conjugated anti rabbit antibody, by Jackson ImmunoResearch (1 mentions)
Rabbit anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Bca protein assay kit, by R&D Systems (1 mentions)
Rabbit anti stat3, by Cell Signaling Technology (1 mentions)
Protein lysis buffer, by Bio-Rad (1 mentions)
Rabbit anti pstat3, by Cell Signaling Technology (1 mentions)
3 protocols using rabbit anti perk5
1
Biochemical analysis of Nfatc1Cre;Krit1fl/fl hearts
2
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein was extracted using RIPA buffer (Sigma-Aldrich) for Western Blot. Protein concentration was measured with using BCA assay (DAKO). Primary antibodies for Western blot are rabbit anti-ERK5 (Cell signaling, San Jose, CA, USA), rabbit anti-pERK5 (Cell signaling) and rabbit anti-GAPDH (Santa Cruz Biotechnology, Dallas, Texas, USA). Secondary antibody is HRP-conjugated anti-rabbit antibody (Jackson ImmunoResearch Labs). Images shown in the figure were representative from 5 repeats. Densitometry of Western blots was quantified with Image J software (NIH, Bethesda, MA, USA).
3
Western Blot Analysis of ERK5 and STAT3
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
As described previously [14 (link),25 (link),26 (link)], the cultured cells were homogenized in protein lysis buffer (Bio-rad, Beijing, China), followed by protein concentration assessment with a BCA protein assay kit (R&D systems, Beijing, China). Western blot was performed with the following primary antibodies: rabbit anti-ERK5 (Cell signaling, San Jose, CA, USA), rabbit anti-pERK5 (Cell signaling), rabbit-anti-Stat3 (Cell Signaling) and rabbit anti-pStat3 (Cell Signaling). The secondary antibody was HRP-conjugated anti-rabbit (DAKO, Beijing, China). Image acquisition and densitometric analysis of the gels were performed with NIH ImageJ software (Bethesda, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!