Solulyse m

Manufactured by Genlantis

Solulyse-M is a laboratory equipment designed for cell lysis and protein extraction. It utilizes a mechanical disruption technique to efficiently break down cell membranes and release cellular contents, including proteins, for further analysis and processing.

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Lab products found in correlation

Ultrascan ccd camera, by Ametek (1 mentions) Tecnai t12, by Thermo Fisher Scientific (1 mentions) Formvar carbon 200 mesh grids, by Ted Pella (1 mentions) Enhanced chemiluminescence system, by Thermo Fisher Scientific (1 mentions) Iblot machine, by Thermo Fisher Scientific (1 mentions) Millipore extraction kit, by Merck Group (1 mentions) Invitrogen buffer wash, by Thermo Fisher Scientific (1 mentions) Westernbreeze chemiluminescent immunodetection kit, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Solulyse (6 citations)

2 protocols using solulyse m

Transmission Electron Microscopy of Cells

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For TEM, cells were cultured in six-well plates in 2 ml of media. Then, 1 µg ml⁻¹ of doxycycline was added to the wells at indicated times with daily media plus doxycycline changes thereafter until harvest. Cells were lysed by adding 400 µl of Solulyse-M (Genlantis) supplemented with 25 U ml⁻¹ of Benzonase Nuclease (Novagen) directly to the six-well plates and incubating for 1 hour at 4 °C with agitation. The lysates were then transferred to 1.5-ml microcentrifuge tubes. Then, 800 µl of 10 mM HEPES (pH 7.5) was added to each tube, and lysates were centrifuged overnight at 300×g and 8° C. Next, 30 µl of the supernatant was collected from the surface on the side of the tube facing the center of the centrifuge rotor and transferred to a new tube. Then, 3 μl of each sample was loaded onto freshly glow-discharged (Pelco EasiGlow, 15 mA, 1 minute) formvar/carbon 200 mesh grids (Ted Pella) and blotted after 1 minute and then air-dried. The unstained grids were imaged on a FEI Tecnai T12 transmission electron microscope equipped with a Gatan Ultrascan CCD camera.

Hurt R.C., Buss M.T., Duan M., Wong K., You M.Y., Sawyer D.P., Swift M.B., Dutka P., Barturen-Larrea P., Mittelstein D.R., Jin Z., Abedi M.H., Farhadi A., Deshpande R, & Shapiro M.G. (2023). Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria. Nature Biotechnology, 41(7), 919-931.

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Protein Expression Profiling in Prostate Cancer

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1 × 10⁶ PC-3 and E006AA prostate cancer cells were cultured for 24 hours, washed with cold PBS, and then lysed with SoluLyse-M (Genlantis, San Diego, CA) cell lysis Tris sucrose buffer. In addition to the cells, total protein was isolated from frozen prostate tumors and homogenized using the Millipore extraction kit (Millipore Corporation, Bilerica, MA). Proteins (30 and 50 μg) were separated using 10% or 16% pre-cast Tris-Glycine gels and dry-transferred for seven minutes using iBlot machine (Invitrogen, Gaithersburg, MD) onto PVDF membranes (Invitrogen, Gaithersburg, MD). The membrane was blocked using WesternBreeze Chemiluminescent Immunodetection Kit (Invitrogen) and probed with anti-Hsp27, Hsp40, Hsp60, Hsp70, Hsp90, NF-κB p65, VEGF, p53 (Ser15), p21 and cyclin D1 (1:500 diluted in manufacturer primary antibody diluent buffer) overnight at 4 °C. After washing with Invitrogen buffer wash (Invitrogen), the blots were treated with either Invitrogen Alk-Phos conjugated (anti-Mouse) or (anti-rabbit) for 30 minutes and washed several times. Proteins were detected by the enhanced chemiluminescence system (Invitrogen). Western analysis was performed on total cell lysate.

Ignacio D.N., Mason K.D., Hackett-Morton E.C., Albanese C., Ringer L., Wagner W.D., Wang P.C., Carducci M.A., Kachhap S.K., Paller C.J., Mendonca J., Li-Ying Chan L., Lin B., Hartle D.K., Green J.E., Brown C.A, & Hudson T.S. (2019). Muscadine grape skin extract inhibits prostate cancer cells by inducing cell-cycle arrest, and decreasing migration through heat shock protein 40. Heliyon, 5(1), e01128.

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