2-Pentanone, diacetyl, acetoin, 2-undecanone, butyl acetate, methyl hexanoate, methyl caprate, ethyl caprate, ethyl butyrate, ethyl hexanoate, 3-methylbutanal, benzaldehyde, pentanal, nonanal, octanal, acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, hexanoic acid, octanoic acid, decanoic acid, valeric acid, δ-dodecalactone, (Z)-dairy lactone, δ-caprinolactone, γ-dodecalactone, dichloromethane and n-alkane standards (C6–C30) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 2-Octanol (internal standard, IS) was purchased from Dr Ehrenstorfer GmbH, Augsburg, Germany. All of the chemicals were of chromatographic grade and had a purity>98 %.
Ethyl caprate
Manufactured by Merck Group
Sourced in United States
Ethyl caprate is a chemical compound that functions as a laboratory reagent. It is the ethyl ester of capric acid, a saturated fatty acid. Ethyl caprate is used in various chemical and scientific applications, but a detailed description of its specific functions or intended uses cannot be provided in an unbiased and factual manner within the constraints of this request.
Automatically generated - may contain errors
Lab products found in correlation
Ethyl hexanoate, by Merck Group (2 mentions)
Ethyl butyrate, by Merck Group (2 mentions)
Benzaldehyde, by Merck Group (1 mentions)
Butyric acid, by Merck Group (1 mentions)
Isovaleric acid, by Merck Group (1 mentions)
Valeric acid, by Merck Group (1 mentions)
N alkane, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Octanal, by Merck Group (1 mentions)
Isobutyric acid, by Merck Group (1 mentions)
Nonanal, by Merck Group (1 mentions)
Octanoic acid, by Merck Group (1 mentions)
Propionic acid, by Merck Group (1 mentions)
Decanoic acid, by Merck Group (1 mentions)
Hexanoic acid, by Merck Group (1 mentions)
2 undecanone, by Merck Group (1 mentions)
Butyl acetate, by Merck Group (1 mentions)
Methyl hexanoate, by Merck Group (1 mentions)
Pentanal, by Merck Group (1 mentions)
3 methylbutanal, by Merck Group (1 mentions)
5 protocols using ethyl caprate
1
Volatile Compounds Analysis Protocol
2
Headspace SPME-GC-MS Analysis of Tea Volatiles
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The analysis of volatiles was performed using a previous method by Li et al. (Li et al., 2020 (link)). About 0.2 g of tea leaves or flower samples was mixed with 5 ml of boiling water in sealed headspace vials and then kept in a water bath kettle at 50°C. After being in equilibrium for 10 min, an SPME fiber was exposed to the sample headspace for 40 min. The volatile compounds were desorbed at the GC-MS injector for 5 min at 230°C. Then, the analysis process of GCMS-QP2010S was performed to detect the compounds as described by Feng et al. (2019) (link). All reference compounds used in this study were well described by Feng et al. (2019) (link). Ethyl caprate (0.2 μg/g, Sigma, USA) was used as the internal standard to normalize the contents of the compounds.
3
Volatile Ester Compound Isolation
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Ethyl acetate, ethyl 2-methylpropionate, propyl acetate, ethyl propionate, ethyl butyrate, isobutyl acetate, ethyl 2-methylbutyrate, ethyl 3-methyl butyrate, isoamyl acetate, ethyl valerate, butyl butyrate, ethyl hexanoate, isoamyl butyrate, propyl hexanoate, ethyl heptanoate, ethyl lactate, 3-methylbutyl hexanoate, propyl octanoate, ethyl nonanoate, hexyl hexanoate, ethyl 2-furoate, ethyl caprate, ethyl benzoate, ethyl phenylacetate, ethyl laurate, ethyl 3-phenylpropanoate, ethyl tetradecanoate, ethyl pentadecanoate, ethyl palmitate, ethyl oleate, and linoleic acid ethyl ester were obtained from Sigma-Aldrich (Shanghai, China). The internal standard (IS) was 2-octanol (Sigma-Aldrich, Shanghai, China). The linear retention index (RI) was determined with a C7–C30 n-alkane mixture (Sigma-Aldrich, Shanghai, China). All of the reagents used were of analytical grade with a purity of at least 97%, and most with a purity exceeding 99%. A Milli-Q purification system provided pure water (Millipore, Bedford, MA, USA). Sodium chloride (analytical grade) and absolute ethanol (analytical grade) were obtained from Sino-pharm Chemical Reagent Co., Ltd (Shanghai, China).
4
Quantifying Cotton Plant Volatiles
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To collect cotton plant volatiles, dynamic headspace sampling method was carried out. One MeJA-exposed or control plant in pot were randomly placed within a glass jar at 18 h after onset of MeJA. The container was sealed with a glass lid that had an air inlet and an air outlet. Air was purified by passage through an activated charcoal filter and pumped through the container at a flow rate of 1500 mL/min with a vacuum pump (Beijing Institute of Labor Instrument, Beijing, China). Eight mm diameter glass tubes comtaining 50 mg of 60/80 mesh Tenax TA (Shanghai ANPEL Science Instrument Company, Shanghai, China) directly connected to the outlet were used to collect volatiles. The collection of volatiles for each treatment was repeated 3 times.
The collected volatiles were extracted with 300 μL of hexane (Fisher, Fairlawn, NJ). 8.65 ng/μL of ethyl caprate (Sigma-Aldrich, Oakville, Canada) was individually added in each extracted sample as an internal standard. Except for the following settings: the GC oven temperature program was 40°C (1-min hold) followed by a rise to 130°C at a rate of 4°C/min (5-min hold) and then to 250°C at a rate of 10°C/min (5-min hold), the rest of GC-MS analysis was executed as mentioned above.
The collected volatiles were extracted with 300 μL of hexane (Fisher, Fairlawn, NJ). 8.65 ng/μL of ethyl caprate (Sigma-Aldrich, Oakville, Canada) was individually added in each extracted sample as an internal standard. Except for the following settings: the GC oven temperature program was 40°C (1-min hold) followed by a rise to 130°C at a rate of 4°C/min (5-min hold) and then to 250°C at a rate of 10°C/min (5-min hold), the rest of GC-MS analysis was executed as mentioned above.
5
Standardized FAME and Alkane References
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Sulfuric acid, methanol, butylated hydroxytoluene (BHT), toluene, sodium chloride, anhydrous sodium sulfate, and diethyl ether were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Hexyl hexanoate, fatty acid methyl ester (FAME), and alkane reference standards were purchased from J&K Chemical Ltd. (Shanghai, China). Margaric acid and ethyl caprate were purchased from Sigma-Aldrich (Steinheim, Germany).
Thirty-seven FAME reference standards with concentrations between 100 ng/mL and 200 ng/mL (C4–C24) were mixed, while FAME reference standards with carbon numbers of C16-C18 were prepared individually, and the purity was 98–99%. The alkane reference standards (C4–C40) were dissolved in chloroform with a concentration of 500 μg/mL.
Thirty-seven FAME reference standards with concentrations between 100 ng/mL and 200 ng/mL (C4–C24) were mixed, while FAME reference standards with carbon numbers of C16-C18 were prepared individually, and the purity was 98–99%. The alkane reference standards (C4–C40) were dissolved in chloroform with a concentration of 500 μg/mL.
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