Ccctaa 3 peptide nucleic acid fish probe

Manufactured by Eurogentec

Sourced in Belgium

The CCCTAA) 3-peptide nucleic acid FISH probe is a laboratory equipment product designed for use in fluorescence in situ hybridization (FISH) applications. It is a synthetic DNA-like molecule that can bind to complementary nucleic acid sequences and be used to detect and visualize specific genetic targets within cells or tissues.

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Lab products found in correlation

Lds 751, by Merck Group (2 mentions) Fc500 flow cytometer, by BD (1 mentions)

2 protocols using ccctaa 3 peptide nucleic acid fish probe

Flow-FISH Analysis of Telomere Length

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Flow-FISH was carried out as described previously.^{16 (link),22 (link),23 (link)} Briefly, vital sterile frozen mononuclear cells from PB were used for the flow-FISH analysis of TL. Samples were prepared for cell denaturation and mixed with a fluorescein isothiocyanate (FITC)-labeled telomere-specific (CCCTAA) 3-peptide nucleic acid FISH probe (Eurogentec, Liège, Belgium) for DNA hybridization followed by DNA counterstaining with LDS 751 (Sigma). Bovine thymocytes were used as internal controls. All measurements were carried out in triplicates. TL of bovine thymocytes was used to convert the TL of lymphocytes and granulocytes into kilobases (kb). Healthy controls and percentiles were used for age adaptation of TL for flow-FISH as described previously.^{16 (link)}

Tometten M., Kirschner M., Meyer R., Begemann M., Halfmeyer I., Vieri M., Kricheldorf K., Maurer A., Platzbecker U., Radsak M., Schafhausen P., Corbacioglu S., Höchsmann B., Matthias Wilk C., Hinze C., Chromik J., Heuser M., Kreuter M., Koschmieder S., Panse J., Isfort S., Kurth I., Brümmendorf T.H, & Beier F. (2023). Identification of Adult Patients With Classical Dyskeratosis Congenita or Cryptic Telomere Biology Disorder by Telomere Length Screening Using Age-modified Criteria. HemaSphere, 7(5), e874.

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Telomere Length Measurement Techniques

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Flow-FISH analyses for telomere length assessment were conducted as follows^{74 (link)}: samples were prepared for cell denaturation and mixed with a FITC labeled telomere specific (CCCTAA)3-peptide nucleic acid FISH probe (Eurogentec) for DNA-hybridization followed by DNA counterstaining with LDS 751 (Sigma). Bovine thymocytes were used as an internal control. Data acquisition was done with a FC-500 flow cytometer (Becton Dickinson). All measurements were carried out single-blinded in triplicates. Healthy control lymphocytes (104 volunteers) were used for age-adaptation of telomere length. Monochrome multiplex quantitative PCR (MM-QPCR) for determination of telomere length was performed as previously described^{75 (link)} (see Supplementary Data 22 for oligonucleotides). T/S ratios were calculated by dividing the number of copies of the telomere template (T) by the beta-globin template (S)^{75 (link)}.

Schrader A., Crispatzu G., Oberbeck S., Mayer P., Pützer S., von Jan J., Vasyutina E., Warner K., Weit N., Pflug N., Braun T., Andersson E.I., Yadav B., Riabinska A., Maurer B., Ventura Ferreira M.S., Beier F., Altmüller J., Lanasa M., Herling C.D., Haferlach T., Stilgenbauer S., Hopfinger G., Peifer M., Brümmendorf T.H., Nürnberg P., Elenitoba-Johnson K.S., Zha S., Hallek M., Moriggl R., Reinhardt H.C., Stern M.H., Mustjoki S., Newrzela S., Frommolt P, & Herling M. (2018). Actionable perturbations of damage responses by TCL1/ATM and epigenetic lesions form the basis of T-PLL. Nature Communications, 9, 697.

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