Testicular level of superoxide dismutase (SOD) was estimated using rat ELISA assay kit (Cusabio Biotech Company, Wuhan, China). Testicular levels of catalase (CAT), glutathione peroxidase (GPx) and nitric oxide (NO) were measured with rat ELISA assay kits ((MyBioSource, San Diego, CA, United States) following the manufacturer’s protocol. Testicular levels of malondialdehyde (MDA), the lipid peroxidation marker, were measured by commercially available rat ELISA kits (Elabscience, Wuhan, China) according to the manufacturer’s instructions. Testicular and seminal total antioxidant capacity (TAC) were assessed using Cell Biolabs OxiSelect™ assay kits (San Diego, Inc., CA, USA), according to the manufacturer’s instructions.
Rat elisa assay kit
Manufactured by Cusabio
Sourced in China
The Rat ELISA Assay Kit is a quantitative analytical tool designed to measure the concentration of a specific target analyte in rat samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the analyte of interest.
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Lab products found in correlation
2 protocols using rat elisa assay kit
1
Testicular Antioxidant Enzyme Evaluation
2
Insulin, Adropin, and Metabolic Markers
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Serum concentrations of insulin and adropin were analyzed with rat ELISA assay kit using the chemiluminescence method (Cusabio, China) according to the instruction of manufacturer by the ELISA microplate reader (Spectrostar Nano, BMG, Labtech, Germany). The serum concentrations of TC, TG, HDL-C, LDL-C, AST, ALT, ALP and GGT activities were measured by using the Beckman Coulter AU680 analyzer (Beckman Coulter, Miami, FL, USA).
For glycosylated hemoglobin A1c (HbA1c,%) measurements, blood samples were collected into 2.0 mL dipotassium (K2) ethylene diamine tetraacetic acid (EDTA) vacuum tubes (BD Vacu-teiner® BD-Plymouth, UK). After blood samples were collected, HbA1C measurement was immediately performed without a delay. Measurement of HbA1C was performed on Tosoh G8 HPLC Analyzer (Tosoh Bioscience, Inc., San Francisco, CA).
Homeostatic model assessment (HOMA-IR) score was calculated using fasting serum insulin and fasting blood glucose concentrations measured at the end of the experimental period according to the following formula: HOMA-IR [(Fasting serum insulin in U/L X fasting blood glucose in mmol/L)/22.5]. β-cell function was quantifi ed with the HOMA-β, with the formula [serum insulin (U/L)×20/glucose (mmol/L) -3.5] (8).
For glycosylated hemoglobin A1c (HbA1c,%) measurements, blood samples were collected into 2.0 mL dipotassium (K2) ethylene diamine tetraacetic acid (EDTA) vacuum tubes (BD Vacu-teiner® BD-Plymouth, UK). After blood samples were collected, HbA1C measurement was immediately performed without a delay. Measurement of HbA1C was performed on Tosoh G8 HPLC Analyzer (Tosoh Bioscience, Inc., San Francisco, CA).
Homeostatic model assessment (HOMA-IR) score was calculated using fasting serum insulin and fasting blood glucose concentrations measured at the end of the experimental period according to the following formula: HOMA-IR [(Fasting serum insulin in U/L X fasting blood glucose in mmol/L)/22.5]. β-cell function was quantifi ed with the HOMA-β, with the formula [serum insulin (U/L)×20/glucose (mmol/L) -3.5] (8).
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